SAB5801049

Alpaca Anti-Chicken IgY single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• 別名: Recombinant Alpaca single variable domain secondary Nano antibody
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: alpaca
• リコンビナント: expressed in E. coli
• 結合体: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: secondary antibodies
• クローン: recombinant monoclonal
• 形状: lyophilized solid
• 分子量: 14 kDa
• 化学種の反応性: Alpaca, chicken
• テクニック: ELISA: 1:10000-1:50000; western blot: 1:10000-1:50000
• サイズ: 0.1 mL
• 色: colorless
• アイソタイプ: VHH
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

Alpaca Anti-Chicken IgY single domain Antibody is designed to specifically detect chicken IgY. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Chicken IgY single domain Antibody detects the chicken IgY selectively but not with mouse, rabbit, human, cynomolgus, rat, goat IgG.
Hens egg yolk immunoglobulins IgY can be transferred from the serum of mother hen to the offspring egg yolk to acquire immunity. In the immunodiagnostic technologies, IgY is an excellent antibody for using in immunological assays involving mammalian sera, due to discriminative properties of IgY compared to mammalian IgG, as IgY does not react with the rheumatoid factor and human anti-mouse IgG antibodies do not activate the complement system and do not bind to Fc receptor. Also, they have poor cross reactivity to mammalian IgG due to immunological differences. IgY does not contain a hinge region but does contain an additional constant domain. The whole IgY molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgYcontains two heavy and light chains. The common IgY is monomeric with a molecular weight of approximately 170 kDa.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

特異性

Fc region of chicken IgY
Isotype: VHH domain of alpaca IgG2b/2c

免疫原

Recombinant Alpaca single variable domain secondary nano antibody

包装

100 μg in Glass bottle

物理的形状

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

再構成

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

保管および安定性

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

その他情報

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317,H412
• 注意書き: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• 危険有害性の分類: Aquatic Chronic 3 - Skin Sens. 1
• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable