SAB4200763

Anti-Dystrophin Antibody

mouse monoclonal, MANDRA1

• 別名: 抗DMD抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 製品名: Anti-Dystrophin antibody, Mouse monoclonal, clone MANDRA1, purified from hybridoma cell culture
• 由来生物: mouse
• 品質水準: 200
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: MANDRA1, monoclonal
• フォーム: buffered aqueous solution
• 分子量: ~427 kDa
• 交差性: zebrafish, rat, mouse, human
• 強化検証: independent; Learn more about Antibody Enhanced Validation
• 濃度: ~1.0 mg/mL
• テクニック: immunoblotting: suitable; immunofluorescence: suitable; immunohistochemistry: 10-20 μg/mL using acetone fixed rat tongue frozen sections
• アイソタイプ: IgG1
• UniProtアクセッション番号: P11532
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... DMD(1756)

詳細

詳細

Anti-Dystrophin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the MANDRA1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Dystrophin is a rod-shaped cytoskeletal protein located to the periphery (plasma membrane) of normal striated muscle fibers.

Dystrophin is encoded by the gene mapped to human chromosome Xp21.2-p21.1.

特異性

Anti-Dystrophin antibody, Mouse monoclonal specifically recognizes an epitope located on the 128 amino acids at the end of the C-terminal domain of the human Dystrophin molecule (residues 3558-3684). The antibody reacts with Dystrophin from human, mouse, rat and zebrafish origin. The antibody is specific to dystrophin and does not react with -actinin or utrophin.

アプリケーション

Anti-Dystrophin antibody, Mouse monoclonal has been used in:

• immunohistochemistry
• immunoblotting
• immunofluorescence
• enzyme-linked immunosorbent assay (ELISA)

生物化学的／生理学的作用

Dystrophin is absent, reduced or altered because of mutation in Duchenne and Becker muscular dystrophies (DMD/BMD) or in its homologue in mouse. Severe DMD is associated with a marked Dystrophin deficiency whereas patients with the milder form of BMD show less pronounced abnormalities of protein expression.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable