indirect immunofluorescence: 2.5-5.0 μg/mL using SH-SY-5Y cells. western blot: 1.0-2.0 μg/mL using using rat brain extracts (S2 fraction) and NIH3T3 fibroblast cell extracts.
Zinc finger protein 746, also known as PARIS, parkin interacting substrate (ZNF746 ) is a 644 amino acid protein. It has a Kruppel-associated box (KRAB) at its N-terminal and a C2HC/C2H2 type zinc finger at its C terminals. This protein is highly conserved among human, mouse and rat, and widely expressed in many tissues.
免疫原
synthetic peptide corresponding to an internal sequence of human ZNF746, conjugated to KLH. The corresponding sequence is identical in human ZNF746 isoform 2, and in mouse and rat ZNF746.
アプリケーション
Anti-ZNF746 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
生物化学的／生理学的作用
Zinc finger protein 746, also known as PARIS, parkin interacting substrate (ZNF746 ) accumulates in Parkinson′s disease (PD) models and in human PD brain. Hence it play a role in the progress of PD disease. ZNF746 is also regulated by the E3 ubiquitin ligase, parkin. In addition, overexpression of ZNF746 leads to the selective loss of dopaminergic neurons in the substantia nigra.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責事項
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Zinc finger protein 746 promotes colorectal cancer progression via c-Myc stability mediated by glycogen synthase kinase 3 beta and F-box and WD repeat domain-containing 7
A hallmark of Parkinson's disease (PD) is the preferential loss of substantia nigra dopamine neurons. Here, we identify a new parkin interacting substrate, PARIS (ZNF746), whose levels are regulated by the ubiquitin proteasome system via binding to and ubiquitination by
