Following disruption using Product No. RTN9604 - GenElute™ 96 Well Total RNA Purification Kit, the samples can be stored at -70°C in Lysis Buffer. Frozen samples are stable for up to 6 months. Before commencing the RNA isolation, frozen samples in Lysis Buffer should be completely thawed and centrifuged.
RTN9604
GenElute™ 96ウェル トータルRNA 精製キット
より環境に優しい選択肢
4 x 96 well plate, sufficient for 4 96-well plate purifications
別名:
High Throughput Purification, RNA Purification
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
1 kit | カートの在庫状況を確認する | ￥335,000 |
この商品について
NACRES:
NA.52
UNSPSC Code:
41105501
￥335,000
カートの在庫状況を確認する
高品質はそのまま、さらにお手頃な価格になりました
usage
sufficient for 4 96-well plate purifications
quality
4 x 96 well plate
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
RNA purification: suitable
input
mammalian cells
mammalian tissue
test parameters
: ~70 min kit run time per plate, sample volume: up to 10^7 cells or 30 mg of tissue
greener alternative category
storage temp.
15-25°C
General description
シグマのGen Elute 96 Well Total RNA Purification Kitは、細胞や組織からtotal RNAを簡便に分離することができます。シリカメンブレン法のメリットを利用しているため、フェノールやクロロホルムなど危険な有機化合物を使う必要がありません。
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
The purified total RNA is ready for use in any enzymatic downstream application.
Biochem/physiol Actions
Sigma′s GenElute 96 Well Total RNA Purification Kit provides a simple and convenient way to isolate total RNA from cells or tissue. The cells or tissue are lysed in a solution containing large amounts of chaotropic salt which immediately inactivates RNases that are present in almost all biological materials. The addition of a Wash Buffer provides the necessary binding conditions for the RNA to be adsorbed to the silica membrane. The contaminating DNA which may be bound to the silica membrane is removed by directly applying RNase-free DNase. The remaining salts, proteins, and other cellular debris are removed by additional washing steps. The final step is to elute the purified RNA in the RNAase-free water that is supplied in the kit.
Features and Benefits
- ハイスループットなフォーマットにより、RNAの迅速な精製が可能です。
- 真空下でも遠心分離にも使用できます。
- 自動化システムでもマニュアル操作でも使用できます。
- RNAは、あらゆる種類の酵素反応に使用できます。
- 独特のプレートデザインにより、交差汚染のリスクが低く抑えられています。
Other Notes
Application notes:
Method of purification:Silica-membrane technology
Compatible with Manual or automated, vacuum or centrifugation
Efficient recovery of > 200 nt
Typical yield20 μg (2 x 106 HeLa cells),
20 μg (20 mg mouse liver)
Typical concentration50–200 ng/μL
Elution volume50–130 μL
Preparation time70 min/plate
Binding capacity100 μg
RNA ratio28S/18S approx. 2.1
A260/A2801.90–2.10
Typical RIN (RNA > 9 (cells)
integrity number)≥ 7 (tissue)
Sufficient for:
< 107 cultured cells (centrifugation)
< 30 mg tissue (centrifugation)
saliva
Related products:
Prod #Related prod #
RTN9604RTN9602
RTN9602RTN9604
Method of purification:Silica-membrane technology
Compatible with Manual or automated, vacuum or centrifugation
Efficient recovery of > 200 nt
Typical yield20 μg (2 x 106 HeLa cells),
20 μg (20 mg mouse liver)
Typical concentration50–200 ng/μL
Elution volume50–130 μL
Preparation time70 min/plate
Binding capacity100 μg
RNA ratio28S/18S approx. 2.1
A260/A2801.90–2.10
Typical RIN (RNA > 9 (cells)
integrity number)≥ 7 (tissue)
Sufficient for:
< 107 cultured cells (centrifugation)
< 30 mg tissue (centrifugation)
saliva
Related products:
Prod #Related prod #
RTN9604RTN9602
RTN9602RTN9604
Legal Information
GenEluteはシグマアルドリッチの登録商標です。
GenElute is a trademark of Sigma-Aldrich Co. LLC
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当該品目 | |||
|---|---|---|---|
| description 4 x 96 well plate, sufficient for 4 96-well plate purifications | description 4 x 96 well plate, sufficient for 4 96-well plate purifications | description sufficient for 10 purifications | description sufficient for 70 purifications |
| technique(s) RNA purification: suitable | technique(s) DNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable |
| usage sufficient for 4 96-well plate purifications | usage sufficient for 4 96-well plate purifications | usage sufficient for 10 purifications | usage sufficient for 70 purifications |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics - | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals |
| quality 4 x 96 well plate | quality 4 x 96 well plate | quality - | quality - |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| greener alternative category | greener alternative category - | greener alternative category , Aligned | greener alternative category |
キットの構成要素のみ
製品番号
詳細
- RNA Wash Buffer 2, Concentrate
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1
supp_hazards
保管分類
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
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