R4030

Anti-hnRNP-E1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-HNRPX, Anti-PCBP1, Anti-hnRNP-X, Anti-poly(rC) binding protein 1
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~37 kDa
• 化学種の反応性: mouse, human, rat
• 濃度: ~1.0 mg/mL
• テクニック: immunoprecipitation (IP): 5-10 μg using K562 cell extract; western blot: 0.5-1 μg/mL using K562 cell extract
• UniProtアクセッション番号: Q15365
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PCBP1(5093) ; mouse ... Pcbp1(23983) ; rat ... Pcbp1(500242)

詳細

詳細

Heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1)/poly(rC)-binding protein 1 (PCBP1) has five major isoforms, hnRNP-E1-E4 and hnRNP-E2-KL (a splice variant of hnRNP-E2). It is a member of the PCBP family or the triple K homology (KH) domain containing protein family. All isoforms of hnRNPE contain three repeats of the ribonucleoprotein KH domain, designated KH1, KH2 and KH3. hnRNP-E1 and hnRNP-E2 are predominantly nuclear with specific enrichment of hnRNP-E1 in nuclear speckles. Among the hnRNPE family, hnRNP-E1 and hnRNP-E2 share the highest amino acid sequence similarity. PCBP1 is ubiquitously expressed and is located on human chromosome 2p13.3.

特異性

Anti-hnRNP-E1 (C-terminal) (also known as PCBP1/2 and αCP1) specifically recognizes human, mouse, and rat hnRNP-E1.

アプリケーション

Anti-hnRNP-E1 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

生物化学的／生理学的作用

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are important in biological activities such as transcription, pre-mRNA processing, mRNA translation and turnover. hnRNPE proteins are also implicated in mRNA stabilization, translation enhancement and translation silencing. Heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1)/poly(rC)-binding protein 1 (PCBP1) acts as a tumor-suppressive splicing factor. Upregulation of this gene can repress the tumorigenicity of ovarian cancer, prostate cancer, gastric cancer, colon cancer and thyroid carcinoma cells.

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)