R0508

(−)-リボフラビン

acrylamide photopolymerization tested

• 別名: ビタミンB₂, ビタミンG, ラクトフラビン
• 実験式（ヒル表記法）: C₁₇H₂₀N₄O₆
• CAS番号: 83-88-5
• 分子量: 376.36
• Beilstein: 97825
• EC Number: 201-507-1
• MDL番号: MFCD00005022
• UNSPSCコード: 12352200
• PubChem Substance ID: 57654584

特性

• 由来生物: synthetic (organic)
• 形状: powder
• mp: 290 °C (dec.) (lit.)
• 適合性: acrylamide photopolymerization tested
• その他の活性: Protease, none detected
• SMILES記法: CC1=C(C)C=C(N(C[C@H](O)[C@@H]([C@H](O)CO)O)C(C2=N3)=NC(NC2=O)=O)C3=C1
• InChI: 1S/C17H20N4O6/c1-7-3-9-10(4-8(7)2)21(5-11(23)14(25)12(24)6-22)15-13(18-9)16(26)20-17(27)19-15/h3-4,11-12,14,22-25H,5-6H2,1-2H3,(H,20,26,27)/t11-,12+,14-/m0/s1
• InChI Key: AUNGANRZJHBGPY-SCRDCRAPSA-N

詳細

アプリケーション

Riboflavin is suitable as a photopolymerization reagent in PAGE by forming free radicals in aqueous solution in the presence of light. Riboflavin photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen but traces of oxygen allows for leucoflavin to reoxidize with free-radical generation. The catalysts TEMED or DMAPN are commonly added to speed up the free radical formation. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules. Riboflavin is commonly used in the stacking gel for non-denaturing PAGE because native proteins can be sensitive to persulfate ions from ammonium persulfate. Another advantage of riboflavin over ammonium persulfate is that it will not start polymerizing until the gel is illuminated.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)