P4306

血小板由来成長因子-BB ヒト

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 別名: PDGF-BB
• MDL番号: MFCD00213023
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 製品名: 血小板由来成長因子-BB ヒト, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in E. coli
• アッセイ: ≥97% (SDS-PAGE)
• フォーム: lyophilized powder
• 有効性: 1.0-3.0 ng/mL EC₅₀
• 品質: endotoxin tested
• 分子量: 24.3 kDa
• 包装: pkg of 10 μg
• 保管条件: avoid repeated freeze/thaw cycles
• テクニック: cell culture | mammalian: suitable
• 不純物: ≤1 EU/μg
• UniProtアクセッション番号: P01127
• 保管温度: −20°C
• 遺伝子情報: human ... PDGFB(5155)

詳細

詳細

Platelet-Derived Growth Factor (PDGF), a cationic polypeptide, is an important serum mitogen predominantly found in cells of mesenchymal origin.[1][2] PDGF is mapped to human chromosome 7p22.3 and is expressed in smooth muscle cells, endothelial cells and macrophages.[3][2] PDGF exists as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.[4]

アプリケーション

Platelet-Derived Growth Factor-BB human has been used in induction of DNA synthesis and proliferation in low-concentration serum.[5]

生物化学的／生理学的作用

Platelet-derived growth factor (PDGF) is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts.[6] PDGF elicits multifunctional actions in a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes.[4][7] PDGF-BB isomer is highly expressed in tumor, mediating oncogenic pericyte recruitment and is is a key regulator of angiogenesis.[4] PDGF-BB controls bone homeostasis, repair and regeneration.[8] It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins and modulates LDL receptor binding.

その他情報

ヒトPDGFのB鎖からなる二量体

物理的形状

0.2 μmフィルタ-で濾過した溶液の凍結乾燥品

アナリシスノート

生物活性は、BALB/c 3T3細胞のthymidine取り込みに対する用量依存的な促進活性として測定されています。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)