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詳細
The activity of proteins depends on phosphorylation of specific amino acid residues. In applications involving identification of activated cellular proteins, it is important to confirm specificity of antibodies used against a particular phosphorylated amino acid. For such applications, inhibitors of phosphorylated antibodies on specific amino acid residues are suitable. Phosphoserine antibody inhibitor may be used for the specific inhibition of the reactivity of anti-phosphoserine antibodies but not that of anti-phosphothreonine- or anti-phosphotyrosine-specific antibodies.
特異性
O-phospho-L-serine conjugated to bovine serum albumin. Phosphoserine antibody inhibitor specifically blocks the reactivity of anti-phosphoserine antibody. It does not block the activity of anti-phosphothreonine or anti-phosphotyrosine-specific antibodies. The product is useful in immunoblotting and in ELISA.
Working dilution is at least 2 μg/ml by the specific inhibition of the reactivity of Monoclonal Anti-Phosphoserine (Sigma Product No. P3430), used in immunoblotting of rat cerebral cortex extract, and in ELISA.
Working dilution is at least 2 μg/ml by the specific inhibition of the reactivity of Monoclonal Anti-Phosphoserine (Sigma Product No. P3430), used in immunoblotting of rat cerebral cortex extract, and in ELISA.
アプリケーション
Phosphoserine-BSA may be used for specific inhibition of monoclonal anti-phosphoserine (Sigma Product No. P3430) in immunoblotting and ELISA applications, at a working dilution of 2 μg/mL.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
P3717-VAR:
P3717-.2ML-PW:
P3717-BULK:
P3717-.2ML:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Birte Schulenberg et al.
Electrophoresis, 25(15), 2526-2532 (2004-08-10)
Protein phosphorylation plays a vital role in the regulation of most aspects of cellular activity, being key to propagating messages within signal transduction pathways and to modulating protein function. Pro-Q Diamond phosphoprotein gel stain is suitable for the fluorescence detection
Motoyuki Ogawa et al.
Biochemical and biophysical research communications, 304(4), 676-683 (2003-05-03)
Paxillin has been recognized as a focal adhesion adapter protein that participates in the integrin-mediated signaling. An earlier study [Ogawa et al. Biochim. Biophys. Acta 1519 (2001) 235] found that frog paxillin was expressed in the kidney epithelial cell line
Matthew G Vander Heiden et al.
Science (New York, N.Y.), 329(5998), 1492-1499 (2010-09-18)
Proliferating cells, including cancer cells, require altered metabolism to efficiently incorporate nutrients such as glucose into biomass. The M2 isoform of pyruvate kinase (PKM2) promotes the metabolism of glucose by aerobic glycolysis and contributes to anabolic metabolism. Paradoxically, decreased pyruvate
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