ORFBFP
MISSION® TRC3 ORF BFP DNA Control
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| サイズ/SKU | 在庫状況 | 単価 |
|---|---|---|
1 vial | カートの在庫状況を確認する | ￥62,800 |
この商品について
NACRES:
NA.51
UNSPSC Code:
41106609
Concentration:
500 ng/μL
packaging
vial of 20 μL
Quality Segment
concentration
500 ng/μL
application(s)
genome mapping
genomic analysis
shipped in
dry ice
storage temp.
−70°C
General description
The MISSION TRC3 ORF BFP Control Vector is a lentivirus plasmid vector. The vector contains an ORF insert expressing BFP, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole Genome LentiORF Pool.
This positive control can be used for measuring transduction efficiency and optimizing ORF delivery in cell lines that do not survive any puromycin exposure. The BFP Control contains a gene encoding BFP driven by the EF1a promoter. This control provides fast visual confirmation of successful transduction. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively.
In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The MISSION TRC3 ORF BFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
This positive control can be used for measuring transduction efficiency and optimizing ORF delivery in cell lines that do not survive any puromycin exposure. The BFP Control contains a gene encoding BFP driven by the EF1a promoter. This control provides fast visual confirmation of successful transduction. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively.
In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The MISSION TRC3 ORF BFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
Functional Genomics/Overexpression Screening/Target Validation
Legal Information
Use of this product is subject to one or more license agreements. For details, please see the MISSION License Page
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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当該品目 | |||
|---|---|---|---|
| description - | description - | description - | description - |
| concentration 500 ng/μL | concentration 500 ng/μL | concentration 1x106 VP/ml (via p24 assay) | concentration 5x108 VP/ml (via p24 assay) |
| storage temp. −70°C | storage temp. −20°C | storage temp. −70°C | storage temp. −70°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| application(s) genome mapping | application(s) genome mapping | application(s) genome mapping | application(s) genome mapping |
| packaging vial of 20 μL | packaging vial of 20 μL | packaging vial of 200 μL | packaging vial of 200 μL |
保管分類
10 - Combustible liquids
一体何が起きているの？
nwg
引火点 (F)
Not applicable
引火点（°C）
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
ORFBFP:
jan