NA9604

GenElute^™ HP 96-Well Plasmid Miniprep Kit

sufficient for 4 96-well plate purifications

• 別名: Gen Elute, GenElute^™HP プラスミド キット
• UNSPSCコード: 41105501
• NACRES: NA.52

特性

• 使用法: sufficient for 4 96-well plate purifications
• テクニック: DNA purification: suitable
• 保管温度: 15-25°C

詳細

詳細

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure. Bacterial cells are harvested by centrifugation and resuspended in Resuspension Solution containing RNase. The cells are then lysed and neutralized followed by the addition of Binding Solution. The prepared lysate is then transferred to a Sigma HP Filter Plate assembled in a vacuum manifold where it is filtered and collected into a deep well plate. The vacuum manifold is rearranged and the filtered lysate is transferred to the Sigma HP Binding Plate. The plasmid DNA is captured onto the silica based membrane and impurities are removed by successive wash steps. Finally, the binding plate is dried and the plasmid DNA is eluted in Elution Solution.

The GenElute HP 96 Well Plasmid Miniprep Kit offers a simple, rapid, costeffective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.

アプリケーション

シグマのMISSION^®shRNA製品ラインに適合するよう設計されたGenElute HP 96-Well Plasmid Miniprep Kit は、要求度の最も高いトランスフェクションプロトコールに最適化されています。精製されたMISSION shRNAプラスミドDNAは、競合製品で精製されるプラスミドに比べ、クラス最高のウイルス力価が得られます。加えて、制限酵素による分解やシークエンシングなど幅広い分子生物学的用途に適した、精製プラスミドDNAが得られます。

特徴および利点

• Up to 10 μg of high-copy plasmid DNA per well
• Flexibility of both manual and automated formats
• Optimized for production of virus titers from MISSION shRNA plasmid vectors
• No phenol/chloroform extraction or alcohol precipitation required

法的情報

GenElute is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• ピクトグラム: GHS02,GHS08,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H226,H290,H302,H314,H334,H336
• 注意書き: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
• ターゲットの組織: Central nervous system
• 保管分類コード: 3 - Flammable liquids
• 引火点(°F): 77.0 °F
• 引火点(℃): 25 °C