This product has not been tested in serum-free, feeder-free induced pluripotent stem cell culture conditions or on stem cells/induced pluripotent stem cells. The effect on pluripotency is currently unknown. No data are available regarding compatibility with these conditions.
MP0030
LookOut® マイコプラズマ除去キット
別名:
Mycoplasma contamination treatment
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| サイズ/SKU | 在庫状況 | 単価 |
|---|---|---|
1 kit | カートの在庫状況を確認する | ￥56,600 |
この商品について
NACRES:
NA.75
UNSPSC Code:
12352207
￥56,600
カートの在庫状況を確認する
usage
kit sufficient for 5 mL mycoplasma elimination
Quality Segment
packaging
pkg of 1 kit
storage condition
protect from light
technique(s)
PCR: suitable
application(s)
detection
microbiology
storage temp.
2-8°C
General description
本キットは、マイコプラズマ汚染を確実かつ完全に排除する生物剤を組み合わせて構成されています。この根絶手順の最初の処理は、ほとんどのアプリケーションでマイコプラズマを十分に除去します。 第2段階で、追加の抗生物質処理により、残存するマイコプラズマを抑制し不活化します。
Application
LookOut®マイコプラズマ除去キットは、細胞およびウイルス培養におけるモリクテスMollicutes および関連微生物（マイコプラズマMycoplasmaアコレプラズマAcholeplasma、スピロプラズマSpiroplasma、エントモプラズマEntomoplasma）の除去に適しています。本製品は、クラミジアChlamydia 培養におけるマイコプラズマ汚染の処理には効果的ではありません。
LookOut®マイコプラズマ除去キットは、細胞培養からマイコプラズマ汚染を迅速かつ効率的に除去するために開発されました。
Features and Benefits
- 試薬の細胞毒性を最小限に抑制
- 高い有効性
- 正常な細胞特性の保持
Legal Information
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
本製品は、グローバルな殺生物剤規制、例えばUS EPAのFederal Insecticide Fungicide and Rodenticide Act、European Biocidal Products Regulation、カナダのPest Management Regulatory Agency、トルコのBiocidal Products Regulation、韓国のConsumer Chemical Products and Biocide Safety Management Act（K-BPR）などの規制の下での殺生物剤としての使用を意図していません。
1 of 1
当該品目 | |||
|---|---|---|---|
| description - | description sufficient for 50 reactions, sufficient for 150 reactions, suitable for RT-PCR, hotstart | description Ready-to-use wet wipes | description Autofluorescence Eliminator Reagent for IHC. |
| technique(s) PCR: suitable | technique(s) whole genome amplification: suitable, RT-PCR: suitable | technique(s) - | technique(s) immunohistochemistry: suitable |
| application(s) detection | application(s) - | application(s) - | application(s) - |
| usage kit sufficient for 5 mL mycoplasma elimination | usage sufficient for 50 reactions, sufficient for 150 reactions | usage sufficient for 50 or 5x50 set Decontamination by wiping off surfaces (wet wipes) | usage - |
| packaging pkg of 1 kit | packaging pkg of 50 reactions (04655877001), pkg of 150 reactions (04655885001) | packaging pkg of 5 × 50 ea Count (in refill sachets for dispenser box), pkg of 50 ea Count Refill (in dispenser box) | packaging pkg of 50 mL |
| Quality Level 200 | Quality Level - | Quality Level - | Quality Level - |
| storage condition protect from light | storage condition - | storage condition - | storage condition - |
保管分類
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
MP0030-VAR: + MP0030-BULK: + MP0030-1KT: + MP0030-1KT-LBL: + MP0030-1KT-PW:
jan
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Is this elimination reagent compatible with serum-free, feeder-free iPSC culture conditions?
1 回答-
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After I dilute my epithelial cells in the media + primary antibiotic, do I not change the media until they reach 80-90% confluency? I usually change the media on the cells every 2-3 days.
1 回答-
The product information sheet protocol for this product instructs to grow the cells in the initial treatment media until 80–90% confluency before passaging. If a media change is necessary for cell health, it is important to include the elimination reagent in the new media.
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Has this been tested in primary cell lines (i.e. skeletal muscle) is there any interference with the normal cell physiology?
1 回答-
The LookOut Mycoplasma Elimination Kit is designed to efficiently eliminate mycoplasma contamination from cell cultures. This kit uses a combination of biological agents to reliably and completely remove mycoplasma contamination. The initial treatment is generally sufficient for mycoplasma elimination in most applications. A second step involves a follow-up antibiotic treatment to suppress and inactivate any remaining mycoplasma. The reagents have minimal cytotoxic properties, and the development of resistant strains is highly unlikely due to the significantly reduced mycoplasma concentration after the initial treatment. Compared to other products for mycoplasma elimination, inactivation, or suppression, the LookOut Mycoplasma Elimination Kit is more effective and has not been shown to cause changes in normal cell characteristics. It is the least cytotoxic method, with overall cytotoxicity demonstrated to be less than 20% across a diverse group of cell lines
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How does the mechanism of action (MOA) of Product No. MP0030, LookOut® Mycoplasma Elimination Kit, differ from that of BM cyclin?
1 回答-
The key distinction lies in Product No. MP0030, LookOut® Mycoplasma Elimination Kit, which actively eradicates mycoplasma by disrupting the membrane. As a lipopeptide, it identifies cholesterol-rich regions in the membrane (as the concentration of cholesterol in mycoplasma membranes is higher than in eukaryotic cells), creating a pore through which the surrounding medium flows into the mycoplasma, ultimately causing it to burst. In contrast, standard antibiotics simply inhibit metabolic pathways, necessitating the rinsing out of mycoplasma through a medium change. If the process is halted prematurely, any remaining mycoplasma can regrow.
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What antibiotics are included in MP0030, the LookOut® Mycoplasma Elimination Kit? Are fluoroquinolones, macrolides, or tetracyclines part of the kit?
1 回答-
The MP0030 product contains two mycoplasma-active antibiotics, with one of them being a fluoroquinolone.
役に立ちましたか？
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