MGECKO2A

Gecko2 Mouse Whole Genome CRISPR Pool, All-in-One^™ Lenti Particles (Gecko2 vector)

• UNSPSCコード: 12352200
• NACRES: NA.51

特性

• 包装: pkg of 8x25 μL (vials)
• 品質水準: 200
• 濃度: 5x10⁸ VP/ml (via p24 assay)
• アプリケーション: CRISPR
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

Developed by Feng Zhang′s lab at the Broad Institute, the mouse GeCKO v2 (all-in-one vector) libraries consist of over 100,000 unique gRNAs for gene knock-out in the mouse genome. Using an all-in-one lenti CRISPR vector which co-expresses Cas9 , gRNA with a puromycin selection marker, the version 2 vector produces nearly 10X higher titer than version 1. Sigma′s lentiviral mouse GeCKO pool all-in-one is provided in 8 x 25 ul aliquots at a minimum titer of 5X10^8 TU/ml (measured by a p24 assay).

Each species-specific library is delivered as two half-libraries (A and B). It is recommended to screen both A and B libraries together, which will include 6 sgRNAs per gene (3 sgRNAs in each library). Both libraries contain 1000 non-targeting control sgRNAs. The A library also targets miRNAs (4 sgRNAs per miRNA).

アプリケーション

Functional Genomics/Screening /Target Validation

特徴および利点

• Use CRISPR nucleases to knockout protein-coding genes to assess their function
• Efficiently screen the whole human genome (16,000+ genes) at the bench-top without robotics or specialized equipment

Numerous built-in enrichment and depletion controls allow researchers to confidently gauge the success of their pooled screening experiments

• Use the one vector system for the mouse GeCKO version 2 libraries in applications where the Cas9 cannot be introduced into mouse cells (like in primary cells).
• Lentiviral CRISPRs can infect a broad variety of mammalian cells by co-expressing a mammalian codon-optimized Cas9 nuclease along with a single guide RNA (sgRNA) to facilitate gene knockout.
• Use puromycin to select after transduction.

調製ノート

Puro Kill Curve and Determining CFU (Colony Formation Unit) per mL. Prior to performing a library-scale screening, two preliminary experiments must be conducted. Visit Sigma.com/pooledscreening.

その他情報

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

法的情報

CRISPR Use License Agreement

Lentiviral License Agreement

All-in-One is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable