MBD0015

4′,6-ジアミジノ-2-フェニルインドール 二塩酸塩

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

• 別名: 2-(4-アミジノフェニル)-6-インドールカルバミジン 二塩酸塩, DAPI 二塩酸塩
• 実験式（ヒル表記法）: C₁₆H₁₅N₅ · 2HCl
• CAS番号: 28718-90-3
• 分子量: 350.25
• Beilstein: 4894417
• MDL番号: MFCD00012681
• UNSPSCコード: 41105500
• NACRES: NA.55

特性

• 品質水準: 200
• 形状: liquid
• 保管条件: protect from light
• 濃度: 1 mg/mL
• テクニック: transfection: suitable
• 溶解性: water: soluble
• 蛍光検出: λ_ex 340 nm; λ_em 488 nm (nur DAPI); λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)
• 適合性: suitable for nucleic acid staining
• 輸送温度: dry ice
• 保管温度: −20°C
• SMILES記法: Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• InChI Key: FPNZBYLXNYPRLR-UHFFFAOYSA-N

詳細

詳細

DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.

アプリケーション

DAPIはアガロ-スゲル中の臭化エチジウムによるDNA染色よりも数倍高い感度を示します。DNAの蛍光フットプリンティングにも使用可能で、また、二本鎖形成を特異的に可視化するためブロッティングの際にアニ-リングしているプロ-ブを検出でき、フロ-サイトメトリ-を使用することでアポト-シス中のDNA変化の調査やDNA量の分析が可能です。DAPI染色は、マイコプラズマに対する高感度かつ特異的な検出方法であることも示されています。

DAPI Readymade Solution is provided at 1mg/mL concentration. It is recommended to dilute DAPI readymade solution 1:1000 with a desired buffer for a 1μg/mL working concentration (typical working concentration used in most applications is 0.1μg/mL-10μg/mL). Store DAPI solution (1mg/mL) -20°C protected from light. It is recommended to aliquot DAPI readymade solution and to avoid freeze thaw cycles.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).

生物化学的／生理学的作用

細胞透過性を有し、副溝に結合するDNA用蛍光プロ-ブです。二本鎖DNAの副溝に(ATリッチなDNAを優先して)結合し、DAPI単独よりも約20倍強い蛍光を発する安定な複合体を形成します。

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

特徴および利点

• Ready-to-use liquid, cell permeable dye.
• Can be used with live cells and fixed cells.
• Effective for fixed-cell staining and quantitation of DNA content.
• Optimal Counterstain when using fluorescent antibodies during fluorescence microscopy or high-content screening (HCS) applications.

物理的形状

DAPI readymade solution is provided at 1mg/mL concentration in water

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable