MAK554

Fluorimetric Acetylcholinesterase Assay Kit

sufficient for 200 fluorometric tests

• UNSPSCコード: 41116104
• NACRES: NA.21

特性

• 検出方法: fluorometric
• 関連疾患: hematological disorder; neurological disorders
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a high catalytic activity- each molecule of AChE degrades about 5000 molecules of acetylcholine per second. Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms, which possess similar catalytic properties, but differ in their oligomeric assembly and mode of attachment to the cell surface.

原理

The Fluorimetric Acetylcholinesterase Assay Kit provides a sensitive method for detecting AChE activity. The kit uses a green substrate to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE. The fluorescence intensity of the substrate is proportional to the formation of thiolcholine, thus the AChE activity.

安全性情報

• ピクトグラム: GHS05
• シグナルワード: Danger
• 危険有害性情報: H318
• 注意書き: P280 - P305 + P351 + P338
• 危険有害性の分類: Eye Dam. 1
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): 188.6 °F - closed cup
• 引火点(℃): 87 °C - closed cup