MAK348

Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric)

sufficient for 100 fluorometric tests

• 別名: Fluorometric Gelatinase Activity Kit
• UNSPSCコード: 12352200
• NACRES: NA.84

特性

• 使用法: sufficient for 100 fluorometric tests
• 検出方法: fluorometric
• 保管温度: −20°C

詳細

詳細

Gelatinases are a type of matrix zinc-dependent metalloproteases (MMPs) that degrade gelatins and a variety of other extracellular matrix proteins. These enzymes are synthesized as latent zymogens that are activated by proteolysis and inhibited by tissue inhibitors of metalloproteases (TIMPs). Two mammalian gelatinases, Gelatinase A (MMP-2) and Gelatinase B (MMP-9), are critical for basement membrane degradation and are highly upregulated in variety of tumor cells.

特徴および利点

Compatible with high-throughput handling systems.

適合性

Suitable for the detection of gelatinase activity in biological samples such as recombinant protein, tissue, cell lysates, etc

原理

The Gelatinase Activity Assay Kit utilizes a hybrid approach for the detection of gelatinase activity by employing a highly quenched gelatin substrate which upon cleavage by a suitable gelatinase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of gelatinase activity (as low as 0.06 mCDU).

安全性情報

• 危険有害性情報: H412
• 注意書き: P273 - P501
• 危険有害性の分類: Aquatic Chronic 3
• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): 188.6 °F
• 引火点(℃): 87 °C