1.15444

Coomassie Brilliant blue G 250 (C.I. 42655)

for electrophoresis

• 別名: Coomassie Brilliant blue G 250 (C.I. 42655), Acid blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, protein gel stain
• 実験式（ヒル表記法）: C₄₇H₄₈N₃NaO₇S₂
• CAS番号: 6104-58-1
• 分子量: 854.02
• MDL番号: MFCD00078482
• UNSPSCコード: 41105305
• EC Index Number: 228-058-4
• NACRES: NC.01

特性

• 品質水準: 100
• 有効性: >5000 mg/kg LD₅₀, oral (Rat)
• テクニック: protein staining: suitable
• pH: 6.4 (20 °C, 10 g/L in H₂O)
• 溶解性: 40 g/L
• かさ密度: 520 kg/m³
• 保管温度: no temp limit
• InChI: 1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1
• InChI Key: CMGWKYXJIYYESN-UHFFFAOYSA-M

詳細

詳細

Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye. CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.

アプリケーション

Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It has also been used in Bradford protein assay.

アナリシスノート

Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ_max. (buffer pH 7.0): 577 - 584 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.01 g/l; buffer pH 7.0; calc. on dried substance): 450 - 570
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for electrophoresis: passes test

法的情報

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable