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安全性情報

MAK143

Sigma-Aldrich

Fluorometric Intracellular Ros Kit

sufficient for 200 fluorometric tests (green)

別名:

Fluorometric ROS Detection Kit

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1 KIT
￥52,020

￥52,020

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1 KIT
￥52,020

About This Item

UNSPSCコード:
12161503
NACRES:
NA.25

￥52,020

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使用法

sufficient for 200 fluorometric tests (green)

検出方法

fluorometric

関連疾患

cancer; neurological disorders

保管温度

−20°C

関連するカテゴリー

詳細

Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.

アプリケーション

Fluorometric Intracellular Ros Kit has been used in reactive oxygen species (ROS) generation assay.[1]

適合性

This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope.

原理

The Fluorometric Intracellular ROS Assay Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a fluorogenic sensor localized to the cytoplasm, resulting in a fluorometric product (λex = 490/λem = 520 nm) proportional to the amount of ROS present. The assay can be performed in either a 96 or 384 multiwell plate format and read using a fluorescence microscope or a fluorescence microplate reader.

保管分類コード

10 - Combustible liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

毒物及び劇物取締法

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PRTR

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消防法

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労働安全衛生法名称等を表示すべき危険物及び有害物

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労働安全衛生法名称等を通知すべき危険物及び有害物

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カルタヘナ法

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Jan Code

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最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
3760362
3760362
3510274
3510274
3181833

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Lu Wang et al.
Journal of cellular and molecular medicine, 25(7), 3449-3459 (2021-03-05)
Tumour necrosis factor-α (TNF-α), a crucial cytokine, has various homeostatic and pathogenic bioactivities. The aim of this study was to assess the neuroprotective effect of ketamine against TNF-α-induced motor dysfunction and neuronal necroptosis in male C57BL/6J mice in vivo and HT-22
Klara Zglińska et al.
PeerJ, 9, e10760-e10760 (2021-02-09)
Elaeagnus umbellata is a plant commonly used in traditional Asian medicine for its many health benefits and strong antioxidative activity. Its therapeutic potential is believed to be connected to its effect on fibroblasts. This study aimed to investigate E. umbellata
Alberto Sola-Leyva et al.
Journal of materials chemistry. B, 8(34), 7667-7676 (2020-07-25)
Recent studies have shown the potential of magnetic hyperthermia in cancer treatments. However, the underlying mechanisms involved have not been yet fully described. In particular, the cell death related to magnetic hyperthermia observed in cultures incubated with low concentration of
Almira Georgieva et al.
Antioxidants (Basel, Switzerland), 10(10) (2021-10-24)
The wastewater from the distillation of rose oils is discharged directly into the soil because it has a limited potential for future applications. The aim of the present study was to determine in vitro the chromatographic profile, redox-modulating capacity, and
Yushan Xia et al.
PLoS pathogens, 17(9), e1009909-e1009909 (2021-09-04)
The emergence and rapid spread of multi-drug resistant (MDR) bacteria pose a serious threat to the global healthcare. There is an urgent need for new antibacterial substances or new treatment strategies to deal with the infections by MDR bacterial pathogens
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質問

1–5/5 質問  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      役に立ちましたか？

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 回答

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      役に立ちましたか？

  3. Does the fluorescence produced by ROS resist PFA fixation? Is it detergent resistant?

    1 回答

    1. This kit is intended to be used on living cultured cells. It is not recommended for use on paraformaldehyde (PFA) fixed cells or tissue, or on cells or tissue that have been treated with a detergent or surfactant.

      役に立ちましたか？

  4. What is the mechanism for detecting ROS? Specifically, what component in the ROS Detection Reagent enables the detection of ROS upon their formation?

    1 回答

    1. The component A ROS green is a non-conjugated compound that is concealed with a ROS-cleavable blocking group. Upon the ROS reaction, the blocking group is cleaved by ROS to release a strongly fluorescent dye. The ROS production is proportional to the concentration of the fluorophore.

      役に立ちましたか？

  5. Is ROS Detection Reagent(Green) the same as 2′,7′-Dichlorofluorescin diacetate?

    1 回答

    1. The composition information for the ROS Detection Reagent, Green solution is considered prioprietary.

      役に立ちましたか？

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