L7391

モノクローナル抗LIS1抗体 マウス宿主抗体

clone LIS1-338, purified from hybridoma cell culture

• MDL番号: MFCD03457563
• UNSPSCコード: 12352203
• PubChem Substance ID: 24896475
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: LIS1-338, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen 46 kDa
• 化学種の反応性: zebrafish, chicken, rat, human, bovine, mouse
• 濃度: ~2 mg/mL
• テクニック: immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.5-1 μg/mL using a rat brain cytosol preparation
• アイソタイプ: IgG1
• UniProtアクセッション番号: P43034
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• SMILES記法: CCCCC\C=C\C\C=C\CCCCCCCC(=O)OCC(O)COP(O)(O)=O
• InChI: 1S/C21H39O7P/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-21(23)27-18-20(22)19-28-29(24,25)26/h6-7,9-10,20,22H,2-5,8,11-19H2,1H3,(H2,24,25,26)/b7-6+,10-9+
• InChI Key: ZQTAMPRZFOOEEP-AVQMFFATSA-N
• 遺伝子情報: human ... PAFAH1B1(5048) ; mouse ... Pafah1b1(18472) ; rat ... Pafah1b1(83572)

詳細

詳細

Monoclonal Anti-LIS1 (mouse IgG1 isotype) is derived from the LIS1-338 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human LIS1 protein. The LIS1 protein (46 kDa) contains seven WD (tryptophan-aspartic acid) repeats, a motif shared by at least 140 known proteins.

The gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) encodes a protein named LIS1 (lissencephaly-1) that forms the α subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase (PAFAH). The gene is mapped to human chromosome 17p13.3.

特異性

イムノブロッティングにおいて、抗体はリン酸化型のLIS1分子と優先的に反応します(46 kDa)。

免疫原

リコンビナントヒトLIS1タンパク質。

アプリケーション

Monoclonal Anti-LIS1 antibody produced in mouse has been used in protein pulldown assay and immunofluorescence.

生物化学的／生理学的作用

LIS1 is involved in cell regulation, including the β subunits of G-proteins (Gβ). This protein motif is likely to mediate protein-protein interactions. LIS1 is a protein that is highly conserved during evolution; bovine, mouse, and chicken proteins exceed 99% similarity.

The heterotrimeric enzyme, platelet-activating factor acteylhydrolase (PAFAH), catalyzes the cleavage of acetyl group at the SN-2 position of platelet-activating factor. The α subunit encoded by the gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) interacts with tubulin and affects microtubule dynamics. Mutations in this gene have been linked to Miller–Dieker lissencephaly, a human brain malformation characterized by a smooth cerebral surface and a disordered organization of the cortical layers resulting from a defect in neuronal migration.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable