indirect immunofluorescence: 1:100 using HeLa cells microarray: suitable western blot (chemiluminescent): 1:2,000 using whole extract of mouse NIH3T3 and rat PC12 cells western blot (chemiluminescent): 1:5,000 using nuclear extract of human HeLa cells
The gene INCENP (inner centromere protein) encodes an inner centromere protein that forms a component of the chromosomal passenger complex (CPC). It is found to be localized to inner centromeres during mitosis. The CPC also contains Aurora B, survivin, and Borealin.
Anti-INCENP antibody produced in rabbit has been used in western blotting and immunofluorescence.
生物化学的／生理学的作用
Studies in knockout mice established INCENP (inner centromere protein) as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis.
The inner centromere protein encoded by the INCENP gene is phosphorylated by Cdk1 (cyclin dependent kinase 1) in mitosis. It is found to be associated with Plk1 (Polo-like kinase) in a polo-box–dependent manner. Plk1 is a serine/threonine kinase that functions in cell cycle, especially in mitosis. INCEP participates in the localization of Bub1 (Budding uninhibited by benzimidazole 1), a serine/threonine kinase involved in spindle checkpoint, to kinetochore. This, in turn, promotes the recruitment of Plk1 to kinetochores. Phosphorylated INCENP stimulates the activity of Aurora B kinase at centromeres and the central spindle that is important for chromosome segregation and cytokinesis, respectively.
物理的形状
0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
免責事項
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Molecular biology of the cell, 14(8), 3325-3341 (2003-08-20)
The function of the Aurora B kinase at centromeres and the central spindle is crucial for chromosome segregation and cytokinesis, respectively. Herein, we have investigated the regulation of human Aurora B by its complex partners inner centromere protein (INCENP) and
Molecular biology of the cell, 17(8), 3705-3716 (2006-06-09)
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