HLTUD002C

MISSION^® Lenti microRNA Inhibitor

cel-miR-243-3p, Negative Control 2, Sequence from Caenorhabditis elegans with no homology to human and mouse gene sequences

• 別名: Tough Decoy, TuD
• UNSPSCコード: 41106609
• NACRES: NA.51

特性

• 製品種目: MISSION^®
• 形状: liquid
• 濃度: ≥1x10⁶ VP/ml (via p24 assay)
• 成熟配列: CGGUACGAUCGCGGCGGGAUAUC
• Sanger mature/minorアクセッション番号: MIMAT0000299
• SangerマイクロRNAアクセッション番号: MI0000319
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. The lentiviral microRNA Inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) is included, allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells.

• Allows for potent inhibition of the desired miRNA
• Lentiviral delivery format allows for efficient delivery of the inhibitor into a wide variety of cell types
• Enables long-term inhibition without repeat transfection

その他情報

miRBase V19 Mature IDに基づく

法的情報

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable