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Merck

GE28-9365-51

Hisprep Fast Flow 16/10

Cytiva 28-9365-51

別名:

16/10 Hisprep, Hisprep Column, Hisprep Fast Flow Column

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About This Item

UNSPSCコード:
23151817
NACRES:
NA.56
現在、価格および在庫状況を閲覧できません。

包装

pkg of 1 ea

メーカー/製品名

Cytiva 28-9365-51

パラメーター

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

ベッドサイズ

16 mm × 100 mm

ベッド容積

20 mL

カラム内径

16 mm

Matrix

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14(Ni2+-stripped medium.)

動作範囲

3-12(Ni2+-stripped medium.)

キャパシティ

~40 mg binding capacity(histidine-tagged protein)

適合性

suitable for bioprocess medium

関連するカテゴリー

詳細

HisPrep FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

HisPrep FF 16/10 columns are prepacked with Ni Sepharose 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

特徴および利点

  • Optimized for convenient scale-up purification of histidine-tagged proteins.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible leakage of Ni2+.
  • High binding capacity, approx. 40 mg/mL medium.
  • Convenient and time-saving 20 mL prepacked HiPrep format.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiPrep is a trademark of Cytiva
HisPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Environment

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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試験成績書(COA)

Lot/Batch Number

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資料

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

すべて表示

プロトコル

Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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