おすすめの製品
包装
pkg of 1 ea
メーカー/製品名
Cytiva 28-9365-51
パラメーター
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
ベッドサイズ
16 mm × 100 mm
ベッド容積
20 mL
カラム内径
16 mm
Matrix
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14(Ni2+-stripped medium.)
動作範囲
3-12(Ni2+-stripped medium.)
キャパシティ
~40 mg binding capacity(histidine-tagged protein)
適合性
suitable for bioprocess medium
関連するカテゴリー
詳細
HisPrep™ FF 16/10 columns are prepacked with Ni Sepharose™ 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose™ 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
特徴および利点
- Optimized for convenient scale-up purification of histidine-tagged proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Negligible leakage of Ni2+.
- High binding capacity, approx. 40 mg/mL medium.
- Convenient and time-saving 20 mL prepacked HiPrep™ format.
保管および安定性
アナリシスノート
その他情報
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
プロトコル
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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