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形状
liquid
メーカー／製品名
Cytiva 27926701
色
colorless
詳細
Ready-To-Go RT-PCR Beads are stable at room temperature and designed for performing single-tube one-step reverse transcription-PCR. Each room-temperature-stable bead contains M-MuLV Reverse Transcriptase, RNase Inhibitor, buffer, nucleotides, and Taq DNA Polymerase. The only additional reagents required are water, template RNA, and primers. The reagents are optimized for full-length cDNA synthesis to >7.5 kb and optimal sensitivity from PCR.
The Ready-To-Go Bead format significantly reduces the number of pipetting steps, thereby increasing reproducibility of the RT-PCR technique and minimizing risk of contamination and RNA degradation. Ready-To-Go RT-PCR Beads are provided in either thin walled 0.5 ml or 0.2 ml tubes compatible with most thermocyclers. The 0.2 ml tubes come assembled in a 96-well (8 × 12) plate format that allows individual strips of eight tubes to be easily removed. This flexibility allows use of either the entire 96-well plate, strips of eight or individual 0.2 ml tubes. Each package of Ready-To-Go RT-PCR Beads contains: RT-PCR beads, control reactions and pd(N)6 and oligo(dT) cDNA primers.
The Ready-To-Go Bead format significantly reduces the number of pipetting steps, thereby increasing reproducibility of the RT-PCR technique and minimizing risk of contamination and RNA degradation. Ready-To-Go RT-PCR Beads are provided in either thin walled 0.5 ml or 0.2 ml tubes compatible with most thermocyclers. The 0.2 ml tubes come assembled in a 96-well (8 × 12) plate format that allows individual strips of eight tubes to be easily removed. This flexibility allows use of either the entire 96-well plate, strips of eight or individual 0.2 ml tubes. Each package of Ready-To-Go RT-PCR Beads contains: RT-PCR beads, control reactions and pd(N)6 and oligo(dT) cDNA primers.
特徴および利点
Pre-dispensed, freeze-dried beads that include the reagents necessary for one-step reverse transcription-PCR* with high sensitivity and reproducibility.
- Preformulated, predispensed, single-dose, ambient-temperature-stable beads ensure greater reproducibility between reactions, minimize pipetting steps, and reduce the potential for pipetting errors and contamination.
- Optimized as a one-tube, one-step RT-PCR* reaction for both cDNA synthesis and PCR.
- Each lot of RT-PCR Beads is function tested for its ability to generate highly specific PCR products to ensure lot-to-lot reproducibility
- For highly reproducible one-step reverse transcription-PCR.*
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
illustra is a trademark of Cytiva
保管分類コード
12 - Non Combustible Liquids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
This page shows PCR and RT-PCR amplification troubleshooting.
This page shows PCR and RT-PCR amplification troubleshooting.
プロトコル
Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.
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製品に関するお問い合わせはこちら（テクニカルサービス）