GE17-5286-01

HisTrap^™ Fast Flow Crude

Cytiva 17-5286-01, pack of 5 × 5 mL

• 別名: Histidine-tagged liquid chromatography column, FF crude columns, IMAC columns, protein purification columns
• UNSPSCコード: 23151816
• NACRES: NA.56

特性

• 包装: pack of 5 × 5 mL
• メーカー／製品名: Cytiva 17-5286-01
• パラメーター: <20 mL/min flow rate; 42 psi (H₂O at room temperature.)
• ベッドサイズ: 16 mm × 25 mm
• ベッド容積: 5 mL
• カラム内径: 16 mm
• Matrix: 6% cross-linked agarose
• 粒径: 45-165 μm
• 平均直径: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• 動作範囲: 3-12(Ni²⁺-stripped medium.)
• キャパシティ: ~40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.); ~40 mg binding capacity(histidine-tagged protein)
• 適合性: suitable for bioprocess medium

詳細

詳細

HisTrap^™ FF crude columns offer the convenience of simple, one-step purification of histidine-tagged proteins directly from homogenized, unclarified cell lysate by immobilized metal ion affinity chromatography (IMAC).

HisTrap^™ FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow. This prepacked column is intended for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). After thorough cell disruption, it is possible to load the unclarified lysate on the column without precentrifugation and filtration of the sample. Extending the duration of the mechanical treatment of the sample to ensure a more complete lysis is recommended. For optimal results, we recommend first addition of lysozyme and DNase I followed by a mechanical lysis, for example by sonication. The homogenized sample can then be loaded directly on the column without a clarification step which may prevent degradation of the target protein and increase the activity.

Ni Sepharose^™ 6 Fast Flow has low nickel ion (Ni²⁺) leakage and is compatible with a wide range of additives used in protein purification. The special design of the column in combination with the medium, provide fast, simple, and convenient purifications. Short purification time generally minimizes deleterious effects, such as degradation and oxidation of sensitive target proteins, and is therefore of great importance. HisTrap^™ FF crude columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design chromatography systems.

特徴および利点

• Optimized for purification of histidine-tagged proteins directly from homogenized, unclarified cell lysates.
• High binding capacity, approx. 40 mg/mL medium and negligible Ni²⁺ leakage
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
• Reduced sample preparation time, which minimizes risk of protein degradation by proteases.
• No need for centrifugation or filtration of the samples. Direct sample application.
• Reliable purification of histidine-tagged proteins directly from unclarified lysates–simply sonicate and run.
• Reduced sample preparation time, which minimizes risk of protein degradation by proteases
• Simple operation with a syringe, pump, or chromatography system such as AKTAdesign or FPLC^™ System
• Permit rapid yet reliable separations with a minimum of sample preparation and equipment

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

FPLC is a trademark of Cytiva

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS09
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids