GE17-5255-01

HisTrap^™ Fast Flow

Cytiva 17-5255-01, pack of 5 × 5 mL

• 別名: HisTrap FF, His-tagged protein purification column, ready-to-use column
• UNSPSCコード: 23151816
• NACRES: NA.56

特性

• 包装: pack of 5 × 5 mL
• メーカー／製品名: Cytiva 17-5255-01
• パラメーター: <4 mL/min flow rate; 42 psi
• ベッドサイズ: 7 mm × 25 mm
• ベッド容積: 1 mL
• カラム内径: 7 mm
• Matrix: 6% cross-linked agarose
• 粒径: 45-165 μm
• 平均直径: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• 動作範囲: 3-12(Ni²⁺-stripped medium.)
• キャパシティ: ~40 mg binding capacity(histidine-tagged protein)
• 適合性: suitable for bioprocess medium

詳細

詳細

HisTrap^™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose^™ 6 Fast Flow matrix make HisTrap^™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap^® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

特徴および利点

• High binding capacity, approx. 40 mg/mL medium.
• Negligible leakage of Ni²⁺.
• Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiTrap is a registered trademark of Cytiva

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS09
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids