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Merck

GE17-5162-01

HiTrap® Anx Fast Flow (high sub)

Cytiva 17-5162-01, pack of 5 × 1 mL

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

ligand

diethylaminopropyl

詳細

Ion Exchanger Type (value)

包装

pack of 5 × 1 mL

メーカー/製品名

Cytiva 17-5162-01

パラメーター

<4 mL/min flow rate
42 psi

ベッドサイズ

7 mm × 25 mm

ベッド容積

1 mL

カラム内径

7 mm

マトリックス

4% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14

working range

3-13

適合性

suitable for bioprocess medium

詳細

HiTrap® ANX (high sub) FF is prepacked with ANX (high sub) Sepharose Fast Flow, and is a weak anion exchanger for small scale purification of higher molecular weight protein as well as screening of binding and elutions conditions.

アプリケーション

Q, SP, DEAE, and CM Sepharose Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.

The active end of the charged group is the same for DEAE Sepharose Fast FLow and ANX Sepharose Fast FLow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast FLow has a diethylaminoethyl-group bound to the Agarose whilst ANX Sepharose 4 Fast FLow (high sub) has a diethylaminopropyl group attached.

特徴および利点

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High fLow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

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資料

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

プロトコル

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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