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Merck

GE17-3712-22

HIS Mag Sepharose Excel

Cytiva 17-3712-22, pack of 10 × 1 mL

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About This Item

UNSPSCコード:
41106500
NACRES:
NA.56

ligand

IMAC immobilized with Ni

包装

pack of 10 × 1 mL

メーカー/製品名

Cytiva 17-3712-22

パラメーター

Ambient temp. range

マトリックス

highly cross-linked spherical agarose including magnetite

関連するカテゴリー

詳細

His Mag Sepharose Excel are magnetic beads designed for rapid, small-scale purification and screening of his-tagged proteins consisting of an Immobilized Metal Affinity Chromatography (IMAC) resin precharged with nickel ions that are strongly bound to a chelating ligand.

Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells in HyClone HyCell CHO cell culture media.

The base of the matrix is based on Sepharose resin, incorporated with magnetite. His Mag Sepharose is available as 10% slurry in three different pack sizes: 2 x 1 mL, 5 x 1 mL, and 10 x 1mL.

Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.

特徴および利点

  • Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture
  • Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Screen histidine-tagged proteins in parallel with high reproducibility and reliable results
  • Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale
  • Simplify handling using visible and dense Sepharose-based magnetic beads

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% ethanol, 5% medium slurry)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Sepharose is a trademark of Cytiva

ピクトグラム

Environment

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

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資料

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

プロトコル

This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from Cytiva.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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