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Merck

GE17-1128-01

MAbtrap® Kit

Cytiva 17-1128-01, pkg of 1 kit

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About This Item

UNSPSCコード:
13111000
NACRES:
NA.56
現在、価格および在庫状況を閲覧できません。

ligand

recombinant protein G lacking albumin-binding region

包装

pkg of 1 kit

メーカー/製品名

Cytiva 17-1128-01

保管条件

(20% Ethanol)

パラメーター

<4 mL/min flow rate
42 psi

ベッドサイズ

7 mm × 25 mm

ベッド容積

1 mL

カラム内径

7 mm

Matrix

highly cross-linked 6% agarose

平均部品サイズ

34 μm

cleaning

2-9

動作範囲

3-9

キャパシティ

>1 mg binding capacity (lgG/column)

保管温度

2-8°C

関連するカテゴリー

詳細

MAbTrap® is an affinity chromatography kit, with everything required for small-scale IgG purification from ascites fluid, serum, and cell culture supernatants.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

アプリケーション

MAbTrap® Kit is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum, and cell culture supernatants. MAbTrap® Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time-consuming buffer preparation and column packing. The kit includes one HiTrap® column, prepacked with Protein G Sepharose High Performance.

特徴および利点

  • Convenient kit for purification of IgG from monoclonal and polyclonal material using a syringe in less than 15 min.
  • Kit contains one HiTrap® Protein G HP column prepacked with Protein G Sepharose High Performance and enough buffer concentrate for 20 preparations with a syringe.
  • MAbTrap® Kit contains everything necessary to perform a complete one-step purification without using complex equipment.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiTrap is a registered trademark of Cytiva
MAbTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Exclamation mark

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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試験成績書(COA)

Lot/Batch Number

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資料

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

プロトコル

This page shows how to prepare samples for purification with affinity chromatography.

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