おすすめの製品
特徴
wetted part: no
包装
pack of 30 pieces
メーカー/製品名
Cytiva 17-0851-01
保管条件
dry media
ベッドサイズ
14.5 mm × 50 mm
ベッド容積
8.3 mL
カラム内径
15 mm
マトリックス
cross-linked dextran
粒径
>50 μm (dry)
85-260 μm
cleaning
2-13
working range
2-13
適合性
suitable for bioprocess medium
関連するカテゴリー
詳細
The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.
Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol. Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.
特徴および利点
- Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex® G-25 Medium.
- Flexible protocols allow runs by gravity flow or centrifugation.
- Easy to use and equilibrate; apply sample; elute.
- Preparation of the samples in the range of 1.0 to 2.5 mL by gravity flow and 1.75 to 2.5 mL with centrifugation.
- Desalt multiple samples in parallel with a desalting capacity >90%.
- Recovery in the range 70 to >95%.
保管および安定性
法的情報
よく一緒に購入される製品
シグナルワード
Warning
危険有害性情報
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
This page shows how to perform a purification of His-tagged membrane proteins.
プロトコル
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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