FLE250
ホタル発光器抽出物
for ATP determination
別名:
FLE
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|
この商品について
NACRES:
NA.81
UNSPSC Code:
12352202
Form:
solution
現在、価格および在庫状況を閲覧できません。
Quality Segment
form
solution
packaging
pkg of 250 mg dried lanterns
technique(s)
toxicology assay: suitable
storage temp.
−20°C
General description
The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.
Application
Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.
Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.
Biochem/physiol Actions
Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin.
Preparation Note
25 mLの水で再構成すると、バイアルの内容物は0.06 Mリン酸カリウム、pH 7.4、0.02 M MgSO4になります。
Other Notes
Crude luciferin and luciferase
1 of 1
当該品目 | |||
|---|---|---|---|
| description for ATP determination | description pkg of 100 mg | description recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein | description Glow-type luciferase assay for the quantification of firefly luciferase gene expression in mammalian cells with signal half-life of about 3 hours |
| technique(s) toxicology assay: suitable | technique(s) toxicology assay: suitable | technique(s) - | technique(s) cell based assay: suitable |
| form solution | form solid | form buffered aqueous solution | form - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. -65 to -96°C |
| packaging pkg of 250 mg dried lanterns | packaging pkg of 100 mg | packaging - | packaging - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
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保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable