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F9182

FSL-biotin

別名:

FSL-CONJ(1Biotin)-SC2-L1

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サイズ/SKU在庫状況単価
1 mg
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¥66,600

この商品について

実験式(ヒル表記法):
C91H149N17NaO32PS
分子量:
2079.28
NACRES:
NA.32
UNSPSC Code:
12352200

¥66,600


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storage temp.

−20°C

Quality Segment

Application

All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. Cells modified with FSL constructs are known as kodecytes and usually maintain their normal vitality and functionality. FSL-biotin has been specifically designed to create biotinylated live cells (kodecytes) but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces. Biotinylated kodecytes can be reacted with a variety of biotin binding protein constructs including fluorescent labeled avidin and avidinylated beads/solid surfaces.
All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. FSL-biotin has been specifically designed to create biotinylated live cells (kodecytes) but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces.

Other Notes

FSL-biotin is a KODE™ technology construct designed to label hydrophobic surfaces including living cells with biotin. All FSL constructs consist of three essential designable features: a functional component (F), a spacer (S), and a diacyl lipid (L). FSL-biotin is comprised of a monomer of biotin (vitamin B7) representing F, conjugated to a maleimide-bearing carboxymethylglycine based linker (SC2) in turn conjugated to an activated adipate derivative of dioleoylphosphatidylethanolamine (L1).

Legal Information

KODE Constructs are supplied by KODE Biotech Materials Limited under license from KODE Biotech Limited.
KODE is a trademark of KODE Biotech Ltd.

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当該品目
F7558F9807F9307
FSL-biotin

Sigma-Aldrich

F9182

FSL-biotin

FSL-Tyr1 ≥90% (TLC)

Sigma-Aldrich

F7558

FSL-Tyr1

FSL-GB3

Sigma-Aldrich

F9807

FSL-GB3

FSL-A(tri)

Sigma-Aldrich

F9307

FSL-A(tri)

description

-

description

≥90% (TLC)

description

-

description

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


保管分類

11 - Combustible Solids

一体何が起きているの?

WGK 3

引火点 (F)

Not applicable

引火点(°C)

Not applicable



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Lot/Batch Number

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質問

1–7/7 質問  
  1. What is the Department of Transportation shipping information for this product?

    1 回答
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      役に立ちましたか?

  2. May I use serum with the product F9182, FSL-biotin?

    1 回答
    1. We do not recommend diluting the Product F9182, FSL-biotin, in lipid-containing buffers. The FSL will associate with the lipids and reduce the efficiency of insertion into cell membranes.

      役に立ちましたか?

  3. How many cells can I label with the product F9182, FSL-biotin?

    1 回答
    1. The exact number of cells may vary based on cell type and cell condition.  The recommended concentration to use for labeling is 10-100ug/ml (~4.8-48mM), which is mixed in a 1:1 volume with cells.

      役に立ちましたか?

  4. Will the Product F9182, FSL-biotin, label dead cells?

    1 回答
    1. Product F9182, FSL-biotin, is designed to insert into the membranes of live cells. But it can also be used to label fixed cells, enveloped virions, and solid phase surfaces.

      役に立ちましたか?

  5. What should I use to reconstitute Product F9182, FSL-biotin?

    1 回答
    1. We recommend reconstituting 1 mg of Product F9182, FSL-biotin with 0.5 mL of saline or PBS for a concentration of 2 mg/mL.

      役に立ちましたか?

  6. What concentration of F9182, FSL-biotin do I use to label cells?

    1 回答
    1. For cell labeling we recommend using a concentration of 10-100ug/ml of F9182, FSL-biotin diluted in PBS. Label cells with FSL-biotin at a 1:1 volume to volume ratio. The exact concentration may need to be optimized for the cells and the application.

      役に立ちましたか?

  7. How does the storage temperature relate to shipping conditions?

    1 回答
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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