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由来生物
mouse
結合体
biotin conjugate
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
M5, monoclonal
フォーム
buffered aqueous solution
テクニック
western blot (chemiluminescent): 2 μg/mL
アイソタイプ
IgG1
輸送温度
dry ice
保管温度
−20°C
詳細
Anti-FLAG® BioM5 monoclonal antibody is a purified murine IgG1 monoclonal antibody that is covalently attached by hydrazide linkage. It can be detected by avidin or streptadivin conjugates.
アプリケーション
N末端にMet-FLAGペプチドが位置する場合のみ、FLAGペプチドに結合します。結合はCa2+依存性ではありません。細胞質内に発現したMet-FLAG融合タンパク質を、哺乳類の粗細胞抽出物から検出するのに使用できますが、大腸菌で発現させた融合タンパク質には使用できません。
Anti-FLAG® BioM5 monoclonal antibody is useful for Western blotting, microscopy applications and formation of avidin-biotin complexes (ABC) in mammalian and Drosophila cells. Anti-FLAG® BioM5 antibody in combination with an avidin or streptavidin conjugate is the preferred anti-FLAG® antibody for detection of FLAG fusion proteins expressed in mouse cells.
The product binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. It is useful for detecting cytoplasmically expressed Met-FLAG® fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
It can be used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Browse additional application references in our FLAG® Literature portal.
The product binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. It is useful for detecting cytoplasmically expressed Met-FLAG® fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
It can be used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Browse additional application references in our FLAG® Literature portal.
特徴および利点
抗FLAG M5-ビオチン標識は、N末端Met-FLAG融合タンパク質に高親和性を示します。
物理的形状
10 mMリン酸ナトリウム溶液(pH 7.4, 150 mM NaCl, 0.02%アジ化ナトリウム含有)
法的情報
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
F2922-.2MG:
F2922-1MG:
F2922-BULK:
F2922-VAR:
最新バージョンのいずれかを選択してください：
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The objective of this study was to evaluate the specific mechanism(s) by which PPARβ regulates mitochondrial content in skeletal muscle. We discovered that PPARβ increases PGC-1α by protecting it from degradation by binding to PGC-1α and limiting ubiquitination. PPARβ also
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Post-translational modifications including protein ubiquitination regulate a plethora of cellular processes in distinct manners. RNA N6-methyladenosine is the most abundant post-transcriptional modification on mammalian mRNAs and plays important roles in various physiological and pathological conditions including hematologic malignancies. We previously
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