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由来生物
bacterial (Leuconostoc mesenteroides)
品質水準
形状
lyophilized powder
比活性
≥100 units/mg protein
組成
Protein, ~15% Lowry
溶解性
H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow
保管温度
−20°C
詳細
Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.
アプリケーション
Dextran Sucrase from Leuconostoc mesenteroides has been used:
- in immobilization on shirasu porous membrane (SPG) for dextran production
- in the enzymatic synthesis of dextran nanoparticles at various pH range
- in the immobilization with hydroxyapatite for dextran production
Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.
The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.
生物化学的／生理学的作用
Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages. The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad. The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.
品質
クロマトグラフィ-精製済み
単位の定義
1ユニットは、pH 5.2、30°C、1分間で、1.0 μmolのフルクトースをスクロースから遊離させる酵素量です。
物理的形状
凍結乾燥品(デキストラン, MESバッファ-塩, CaCl2を含有)。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
D9909-10UN:
D9909-VAR:
D9909-50UN:
D9909-10UN-PW:
D9909-BULK:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Ah-Rum Yi et al.
Journal of microbiology and biotechnology, 19(8), 829-835 (2009-09-08)
A dextransucrase (LcDS) gene from Leuconostoc citreum HJ-P4 has been amplified and cloned in E. coli. The LcDS gene consists of 4,431 nucleotides encoding 1,477 amino acid residues sharing 63-98% of amino acid sequence identities with other known dextransucrases from
Bioengineering of Leuconostoc mesenteroides glucansucrases that gives selected bond formation for glucan synthesis and/or acceptor-product synt
Kang, H., et al.
RNA, 59, 4148-4155 (2011)
Hongbin Zhang et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 25(12), 2022-2028 (2010-04-01)
We optimized the medium for recombinant dextransucrase expression in engineering strain Escherichia coli BL21 (DE3)/pET28-dexYG by an Orthogonal experiment. After the medium had been decided, we studied the effect of temperature, sucrose concentration and pH value on the yield. The
Hidetaka Kawakita et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 877(3), 347-350 (2009-01-03)
Dextransucrase forms a complex with dextran during an enzymatic reaction with sucrose. Using its enzymatic character, we performed a continuous and dynamic rejection of colloidal particles by generating dextran with dextransucrase immobilized in an inorganic porous membrane. Inorganic membranes having
Enzymatically synthesized dextran nanoparticles and their use as carriers for nutraceuticals
Semyonov D, et al.
Food & Function, 5(10), 2463-2474 (2014)
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