D9909
デキストランスクラーゼ from Leuconostoc mesenteroides
lyophilized powder, ≥100 units/mg protein
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
10 units | カートの在庫状況を確認する | ￥73,300 |
この商品について
CAS番号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/mg protein
Biological source:
bacterial (Leuconostoc mesenteroides)
￥73,300
カートの在庫状況を確認する
高品質はそのまま、さらにお手頃な価格になりました
biological source
bacterial (Leuconostoc mesenteroides)
Quality Segment
form
lyophilized powder
specific activity
≥100 units/mg protein
composition
Protein, ~15% Lowry
solubility
H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow
storage temp.
−20°C
General description
Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.
Application
Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.
Dextran Sucrase from Leuconostoc mesenteroides has been used:
- in immobilization on shirasu porous membrane (SPG) for dextran production
- in the enzymatic synthesis of dextran nanoparticles at various pH range
- in the immobilization with hydroxyapatite for dextran production
The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.
Biochem/physiol Actions
Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages.[1] The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad.[2] The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.[3]
Physical form
凍結乾燥品(デキストラン, MESバッファ-塩, CaCl2を含有)。
Analysis Note
クロマトグラフィ-精製済み
Other Notes
1ユニットは、pH 5.2、30°C、1分間で、1.0 μmolのフルクトースをスクロースから遊離させる酵素量です。
1 of 1
当該品目 | |||
|---|---|---|---|
| biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) |
| specific activity ≥100 units/mg protein | specific activity - | specific activity - | specific activity - |
| form lyophilized powder | form powder | form powder | form powder |
| storage temp. −20°C | storage temp. - | storage temp. - | storage temp. - |
| solubility H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow | solubility H2O: soluble 20 mg/mL | solubility H2O: 100 mg/mL, clear, colorless | solubility H2O: 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)