This product can be reconstituted in water at 01. mg/mL. The solubility in PBS has not been determined, but is expected to be similar. Please see the link below to review additional preparation instructions included in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/272/751/d8935dat.pdf
D8935
Nidogen-1 (NID1) human
recombinant, expressed in HEK 293 cells, suitable for cell culture
別名:
Entactin, NID, NID-1, NID1
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| サイズ/SKU | 在庫状況 | 単価 |
|---|---|---|
50 μg | カートの在庫状況を確認する | ￥60,100 |
この商品について
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
95% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells
recombinant
expressed in HEK 293 cells
assay
95% (SDS-PAGE)
form
lyophilized powder
technique(s)
cell culture | mammalian: suitable
storage temp.
−20°C
General description
Nidogen-1 belongs to the nidogens family of glycoproteins. This family consists of nidogen-1 and nidogen-2, both of which are sulfated monomeric glycoproteins. Nidogen-1 is ubiquitously present in the synaptic and extra synaptic basal lamina. It is mapped to human chromosome 1q42.3. Nidogen-1 encodes a 139 kDa, three globular domains namely G1, G2 and G3 and has multiple epidermal growth factor repeats. Recombinant human nidogen-1 is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 133.5 kDa (amino acids 29-1247). This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
Application
This recombinant human nidogen-1 product can be used as an attachment factor for cells adherence.
Biochem/physiol Actions
Nidogen-1 (NID-1) is thought to have an important role in the construction of basement membrane by linking between the networks of laminin and collagen IV. Genetic elimination of the nidogen-1 binding site on the mouse laminin γ1 chain leads to impaired deposition of basement membranes and hampers microvessel maturation. Nidogen 1 and 2 double knockout in mouse fibroblasts led to the loss of all major basal membrane components from the epidermal-collagen interface, and complete abolishment of hemidesmosomes. Supplementation by either recombinant nidogen-1 or -2 improved basal membrane formation. Mutations in NID1and laminin γ1 is implicated in pathogenesis of Dandy-Walker spectrum disorders.
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当該品目 | |||
|---|---|---|---|
| description recombinant, expressed in HEK 293 cells, suitable for cell culture | description recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture | description recombinant, expressed in HeLa cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture | description recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture |
| recombinant expressed in HEK 293 cells | recombinant expressed in HEK 293 cells | recombinant expressed in HeLa cells | recombinant expressed in HEK 293 cells |
| assay 95% (SDS-PAGE) | assay ≥97% (HPLC), ≥97% (SDS-PAGE) | assay ≥95% (SDS-PAGE), ≥95% (HPLC) | assay ≥95% (SDS-PAGE) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form lyophilized powder | form lyophilized | form lyophilized | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
保管分類
13 - Non Combustible Solids
一体何が起きているの？
WGK 1
引火点 (F)
Not applicable
引火点（°C）
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
D8935-50UG-PW: + D8935-50UG: + D8935-VAR: + D8935-BULK:
jan
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What is the solubility of this protein in water and PBS?
1 回答-
役に立ちましたか？
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