NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93061524, mouse embryo, Fibroblast
tube of 5 μg 93061524-DNA-5UG pkg of vial of cells 93061524-1VL
成長モード
Adherent
核型
Not specified
形態
Fibroblast
製品
Not specified
受容体
Not specified
テクニック
cell culture | mammalian: suitable
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Mouse Swiss NIH embryo
細胞株の説明
Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.
アプリケーション
DNA transfection studies
NIH 3T3 Cell Line has been used to:
study calcium-mediated actin reset (CaAR) in response to physiological changes[1]
study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation[2]
determine the viral titer of the retroviral construct -ST40hTNFα[3]
培地
DMEM + 2mM Glutamine + 10% Calf Serum (CS).
継代と培養方法
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.
その他情報
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試験成績書（COA）
Lot/Batch Number
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DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
