C8243

モノクロナール抗カテプシンK マウス宿主抗体

~2 mg/mL, clone CL124-1H6, purified immunoglobulin, buffered aqueous solution

• 別名: 抗CTSK抗体, 抗CTSO2抗体, 抗PKND抗体, 抗PYCD抗体
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD02097890
• Conjugate: unconjugated
• Clone: CL124-1H6, monoclonal
• Application: ELISA (i), WB
• Citations: 12

特性

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: CL124-1H6, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~27 kDa
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): indirect ELISA: suitable, western blot: 10-20 μg/mL using total cell extracts of HCT-116 cells
• isotype: IgG2b
• UniProt accession no.: P43235
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CTSK(1513)

詳細

General description

Cathepsins are proteases that normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell. Cathepsins are classified based on their functions and presence of active site such as cathepsin B, D, K, V, F etc.

Monoclonal Anti-Cathepsin K (mouse IgG2b isotype) is derived from the hybridoma CK124-1H6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human cathepsin K.

Immunogen

組み換えヒトカテプシンK。エピト-プは、アミノ酸222~235に相当するペプチド配列RGYREIPEGNEKAL内に存在します。

Application

Monoclonal Anti-Cathepsin K antibody produced in mouse has been used in western blotting and enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

本抗体は、類似したリソソ-ムシステインプロテア-ゼであるカテプシンL、B、H、S、V、Wとは交差反応しません。

Cathepsin is involved in various functions such as intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. Among all the types, cathepsins K (Ctsk) is highly expressed in osteoclasts that plays a crucial role in bone remodeling and resorption. Cathepsin K protein was found in primary breast carcinoma and prostate cancer where it performs in the invasive process of prostate cancers, thyroid carcinoma, lung adenocarcinoma and breast carcinoma. Its deficency causes pycnodysostosis, a rare bone dysplasia with an osteopetrotic-like phenotype.

Physical form

15 mMアジ化ナトリウム含有のpH 7.4の0.01 Mリン酸緩衝食塩水溶液.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)