western blot: 0.5-1.0 μg/mL using HEK-293T cells expressing human calsequestrin-2 western blot: 0.5-1.0 μg/mL using extract of rat cardiac muscle (S1 fraction)
Calsequestrin is a major Ca2+ binding protein in cardiac and skeletal muscle.
Calsequestrin-2 (55 kDa) is found in both sarcoplasmic reticulum′s (SR) terminal cisternae luminal space of both cardiac and in slow skeletal muscle cells. It is made of three domains each with negatively charged thioredoxin-like folds with a β-sheet surrounded by four α-helices.
Anti-Calsequestrin-2 antibody has been used in immunoblotting, western blotting.
Anti-Calsequestrin-2 is suitable for western blot at a concentration of 0.5-1.0μg/mL using HEK-293T cells expressing human calsequestrin-2 and western blot at a concentration of 0.5-1.0μg/mL using extract of rat cardiac muscle (S1 fraction)
生物化学的／生理学的作用
Over expression of cardiac calsequestrin (CASQ2) is associated with depressed cardiovascular function and hypertrophy. Mutations in CASQ2 are thought to be associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). It is a high-capacity, low-affinity Ca2+ binding glycoprotein, which functions as an internal Ca2+ store in the lumen of the sarcoplasmic reticulum (SR).
物理的形状
0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
免責事項
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Tissue engineering. Part A, 20(11-12), 1654-1667 (2014-01-15)
Electrical and mechanical stimulation have both been used extensively to improve the function of cardiac engineered tissue as each of these stimuli is present in the physical environment during normal development in vivo. However, to date, there has been no
Calsequestrin 2 and arrhythmias
Faggioni M and Knollmann B C.
American Journal of Physiology. Heart and Circulatory Physiology, 302(6), H1250-H1260 (2011)
Journal of tissue engineering and regenerative medicine, 11(2), 342-353 (2014-06-12)
Mechanical stimulation has been used extensively to improve the function of cardiac engineered tissue, as it mimics the physical environment in which the tissue is situated during normal development. However, previous mechanical stimulation has been carried out under a constant
Calsequestrins in skeletal and cardiac muscle from adult Danio rerio
Furlan S, et al.
Journal of Muscle Research and Cell Motility, 37(1-2), 27-39 (2016)
