immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10,000 using sections of rat cerebellum microarray: suitable western blot: 1:20,000-1:1,000 using rat brain extract and Jurkat, whole cell extract, respectively
CaM Kinase IV is a Ca2+/calmodulin-dependent kinase that belongs to serine/threonine protein kinases family. CaM Kinase IV is expressed in the brain and thymus and is localized in cellular cytoplasm and nucleus. It facilitates the phosphorylation of several substrates in brain like-synapsin I, microtubule associated protein (MAP2), tau and tyrosine hydroxylase. Anti-caM kinase IV (CaMKIV) (AY-18) antibody can be used in western blotting (diluted 1:1000 and 1: 20,000) using human T-cell leukemia Jurkat whole cell extract and rat brain extract. It can also be used in microarray. Rabbit anti-caM kinase IV (CaMKIV) (AY-18) antibody reacts specifically with CaMKIV of rat but cross reacts with human CaMKIV.
免疫原
Peptide corresponding to amino acids 457-474 of rat CaM kinase IV conjugated to KLH. This sequence has extensive homology to mouse (80%) and human (70%) CaMKIV.
アプリケーション
Anti-caM kinase IV (CaMKIV) (AY-18) antibody (diluted 1:500) can be used as a primary antibody in immunohistochemical studies. It can also be used in immunoblotting to detect and localize CAMKIV (65 kDa).
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The Journal of biological chemistry, 266(26), 17592-17596 (1991-09-15)
The regional and tissue-specific expression of the Ca2+/calmodulin-dependent protein kinase, CaM kinase-Gr, were examined. The Mr 65,000 alpha-polypeptide of CaM kinase-Gr is expressed ubiquitously in different anatomical regions of rat brain, whereas an additional Mr 67,000 beta-polypeptide is observed solely
Pyramidal neurons in rodent visual cortex homeostatically maintain their firing rates in vivo within a target range. In young cultured rat cortical neurons, Ca2+/calmodulin-dependent kinase IV (CaMKIV) signaling jointly regulates excitatory synaptic strength and intrinsic excitability to allow neurons to
We observed the distribution pattern of Ca2+/calmodulin-dependent protein kinase IV in rat brain and spinal cord using an immunohistochemical method by light and electron microscopy. Particularly strong immunoreactivity was detected in the telencephalic structures such as the olfactory bulb, cerebral
The Journal of pharmacology and experimental therapeutics, 309(1), 310-319 (2004-01-14)
Intracellular recording methods with "sharp" microelectrodes were used to study signal transduction mechanisms underlying the excitatory action of bradykinin (BK) in morphologically identified neurons in the small intestinal submucosal plexus. Exposure to BK evoked slowly activating membrane depolarization and enhanced
