A7549

抗アポトーシス誘導因子抗体 ウサギ宿主抗体

IgG fraction of antiserum, buffered aqueous solution

• 別名: 抗AIF抗体
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD02685955
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, WB
• Citations: 12

特性

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 57 kDa
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μL
• technique(s): microarray: suitable, western blot: 1:1,000 using human epitheloid carcinoma HeLa cell extract and mouse brain extract
• UniProt accession no.: O95831
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... AIFM1(9131) ; mouse ... Aifm1(26926)

詳細

General description

Apoptosis-Inducing Factor (AIF) is a mitochondrial flavoprotein that can induce apoptosis in isolated nuclei. Studies have reported that AIF can induce the realease of cytochrome c and caspase 9. Bcl-2 is known to inhibit AIF release without affecting its apoptotic functions.
Rabbit Anti-AIF antibody recognizes human and mouse AIF (57kDa). Staining of AIF in immunoblotting is specifically inhibited with the AIF immunizing peptide (human, amino acids 593-613).

Apoptosis-inducing factor (AIF) is encoded by the gene mapped to human chromosome X (Xq25-Xq26). It is a 57 kDa mitochondrial flavoprotein. AIF contains two mitochondrial localization sequences and two putative nuclear localization sequences.

Immunogen

ヒトアポト-シス誘導因子(AIF)のC末端アミノ酸593-613に相当する合成ペプチドのKLH結合体。

Application

Protein lysates from 2CLL, 10 MCl and Jeko-1 cells were analyzed by western blot using anti-AIF as the primary antibody. Cells also underwent intracellular delivery by being transfected with anti-AIF at a final dilution of 1:2500 and then analyzed by FLOW cytometry.

Rabbit Anti-Apoptosis-Inducing Factor (AIF) antibody has been used for immunoblot analysis at 1:1000 and 1:2000 dilutions. The antibody can also be used for microarray applications.

Biochem/physiol Actions

Microinjection of apoptosis-inducing factor (AIF) into the cytoplasm of intact cells induces chromatin condensation, dissipation of the mitochondrial transmembrane potential and exposure of phosphatidylserine in the plasma membrane.

Physical form

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable