C0581

モノクロナール抗カルシニューリン (β-サブユニット) マウス宿主抗体

clone CN-B1, ascites fluid

• 別名: Anti-CALNB1, Anti-CNB, Anti-CNB1
• MDL番号: MFCD00282169
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: CN-B1, monoclonal
• 含みます: 15 mM sodium azide
• 交差性: bovine, rat, human, mouse
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using neurons in human myenteric and prostatic ganglia; indirect ELISA: suitable; western blot: 1:3,000 using rat brain extract
• アイソタイプ: IgG2b
• UniProtアクセッション番号: P63098
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PPP3R1(5534) , PPP3R2(5535) ; rat ... Ppp3r1(29748) , Ppp3r2(29749)

詳細

詳細

Calcineurin (CaN, heterodimeric enzyme) is a Ca(²⁺)/calmodulin (CaM)-stimulated protein phosphatase 3 (PPP3) consisting of a catalytic A subunit (CnA) and a Ca(²⁺)-binding regulatory B subunit (CnB). The calcium-binding regulatory subunit (calcineurin B) is coded by the PPP3R1 gene located on human chromosome 2. Protein phosphatase 3, regulatory subunit B, α (PPP3R1) is also known as CALN, CCN1, CNA1, CALNA, PPP2B and CALNA1. The regulatory subunit (calcineurin B) is widely expressed in brain while the catalytic subunit (calcineurin A) is abundantly expressed in Hassall′s corpuscles, localized in the thymic medulla and represents the terminal stages of thymic medullary epithelium.

Monoclonal Anti-Calcineurin (β-subunit) (mouse IgM isotype) is derived from the CN-B1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified bovine brain calcineurin preparation. Two different calcineurin B has been reported in rat testis.

特異性

抗体は、イムノブロッティングにおいてカルシニュ-リンのβ-サブユニット(18 kDa, 別名カルシニュ-リンB)に位置するエピト-プを認識しますが、α-サブユニットとの交差反応性はありません。

免疫原

ウシ脳カルシニュ-リン。

アプリケーション

Monoclonal Anti-Calcineurin (b-Subunit) antibody produced in mouse has been used in immunohistochemistry, indirect ELISA and western blotting.

生物化学的／生理学的作用

Calcineurin calcium -binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. immunoreactivity is detected at significant concentrations only in normal and neoplastic neurons regions of the cytoplasm dendrites, axons and axon terminals. A close correlation has been observed between inhibition of calcineurin by the complexes of cyclosporin A/cyclophilin and tacrolimus(FK506) and FK506 binding proteins (FKBPs) and inhibition of apoptosis. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage, such as an ischemic insult.

Calcineurin is a major soluble calmodulin-binding protein in the brain. It stimulates the expression of the surface molecules CD83, CD80, CD86, CD40 and HLA-DR by promoting secretion of inflammatory cytokines IL-6, TNF-α and IL-1β by human PBMC-derived dendritic cells. It plays an important role in signal transduction, activation of T cell. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable