B3275

モノクロナール抗ウサギ免疫グロブリン (IgG, IgA, IgM)

clone RG-16, purified immunoglobulin, buffered aqueous solution

• 別名: Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: biotin conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: secondary antibodies
• クローン: RG-16, monoclonal
• フォーム: buffered aqueous solution
• 化学種の反応性: rabbit
• 以下との反応禁止: human; pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
• テクニック: direct ELISA: 1:60,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500; western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well); western blot: 1:200,000
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

特異性

ウサギIgとのみ結合します。還元されたウサギIgとは反応しません。

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

生物化学的／生理学的作用

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens.

物理的形状

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable