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安全性情報

AC-6000

Sigma-Aldrich

HighPrep™ PCR Clean-up System

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About This Item

UNSPSCコード:
41105500

フォーム

liquid

包装

pkg of 250 mL (AC-60250 (HighPrep™ PCR (250 mL)))
pkg of 5 mL (AC-60005 (HighPrep™ PCR (5 mL)))
pkg of 50 mL (AC-60050 (HighPrep™ PCR (50 mL)))
pkg of 500 mL (AC-60500 (HighPrep™ PCR (500 mL)))

保管条件

do not freeze

保管温度

2-8°C

詳細

Magnetic beads-based reagent for manual and for automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and amplicon size selection.

The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.

アプリケーション

HighPrep™ PCR Clean-up System may be used in:
  • Post-PCR and post-enzymatic reaction clean-up[1][2][3][4][5]
  • NGS library preparation
  • Amplicon size selection for NGS[6]
  • Microarrays
  • PCR
  • Sanger sequencing
  • Restriction enzyme digestions, adapter ligations
  • Cloning

特徴および利点

  • High recovery of >100 bp amplicons
  • Adaptable to high throughput liquid handling systems
  • Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
  • No centrifugation or filtration leads to higher and purer yields

保管および安定性

14 months when stored as directed

法的情報

HighPrep is a trademark of MagBio Genomics, Inc.

免責事項

For Research Use Only. Not for Use in Diagnostic Procedures.

ピクトグラム

Health hazard
GHS08

シグナルワード

Warning

危険有害性情報

H361f

危険有害性の分類

Repr. 2

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

労働安全衛生法名称等を表示すべき危険物及び有害物

名称等を表示すべき危険物及び有害物

労働安全衛生法名称等を通知すべき危険物及び有害物

名称等を通知すべき危険物及び有害物

Jan Code

AC-60500:4548173363561
AC-60250:4548173363554
AC-60005:4548173363530
AC-60050:4548173363547

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試験成績書（COA）

Lot/Batch Number

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Shuba Varshini Alampalli et al.
PloS one, 12(12), e0188603-e0188603 (2017-12-09)
India has the third largest number of HIV-1-infected individuals accounting for approximately 2.1 million people, with a predominance of circulating subtype C strains and a low prevalence of subtype A and A1C and BC recombinant forms, identified over the past
Eva Baggesgaard Sterndorff et al.
Environmental research, 185, 109449-109449 (2020-04-12)
Activity of the microbial population in clothing causes unpleasant odor and textile deterioration. However, little is known about how the textile microbial community is shaped. In this study, we developed a method for extracting DNA from small amounts of detergent-washed
The T-shirt microbiome is distinct between individuals and shaped by washing and fabric type
Sterndorff EB, et al.
Environmental Research, 109449-109449 (2020)
Lauren Bowen Reoma et al.
Annals of neurology, 86(6), 878-884 (2019-10-11)
JC virus (JCV) infection is a lytic infection of oligodendrocytes in progressive multifocal leukoencephalopathy; less common forms of central nervous system manifestations associated with JCV infection include granule cell neuronopathy, encephalopathy, and meningitis. Presented is the first case of fatal
Soil bacterial diversity under conservation agriculture-based cereal systems in Indo-Gangetic Plains
Choudhary M, et al.
3 Biotech, 8, 304-304 (2018)
関連する文献をすべて表示

プロトコル

HighPrep™ PCR Clean Up Kit and Purification System Protocol

HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.

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レビュー

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アクティブなフィルタ

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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