A7554

モノクローナル抗ウシIgG抗体

clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution

• 別名: Monoclonal Anti-Bovine IgG
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 結合体: alkaline phosphatase conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: secondary antibodies
• クローン: BG-18, monoclonal
• 形状: buffered aqueous glycerol solution
• 化学種の反応性: bovine (non-reactive to IgM)
• 以下との反応禁止: pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat
• テクニック: direct ELISA: 1:25,000; dot blot: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG; western blot (chemiluminescent): 1:100,000
• アイソタイプ: IgG1
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.

特異性

bovine IgG1 and IgG2

免疫原

Purified bovine IgG

アプリケーション

Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:

• western blotting
• enzyme linked immunosorbent assay (ELISA)
• dot blot
• immunohistochemistry

生物化学的／生理学的作用

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

物理的形状

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)