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由来生物
goat
結合体
alkaline phosphatase conjugate
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
secondary antibodies
クローン
polyclonal
形状
buffered aqueous solution
化学種の反応性
mouse
以下との反応禁止
bovine, human, horse
テクニック
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:160,000-1:320,000
輸送温度
wet ice
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
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詳細
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation in mice.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
免疫原
Purified mouse IgG, Fc fragment
アプリケーション
PBS/0.1%Tweenおよび1%BSAで1：1000に希釈し、37°Cで1.5時間、ELISAアッセイでアルカリホスファターゼ結合ヤギ抗マウスFc特異抗体を二次抗体として使用しました。37°Cで30分間、基質として4-ニトロフェニルホスフェート（Sigma）を使用して抗体を開発しました。
Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA (1:1500) and western blot. The antibody can also be used for immunohistochemistry (1:40 using formalin-fixed, paraffin-embedded sections).
Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA), sandwich ELISA and enzyme-linked immune absorbent spot (ELISPOT).
The product has been used as a secondary antibody for detecting mouse serum anti-hemagglutinin (HA) antibodies using ELISA techniques.
生物化学的／生理学的作用
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
その他情報
本抗体は、ウシ, ウマ, ヒト血清タンパク質で吸収済です。
物理的形状
0.05M Tris溶液 (pH 8.0, 1%BSA, 1mM MgCl2 , 15mMアジ化ナトリウム含有)
調製ノート
ウシ, ウマ, ヒトサンプルでのバックグラウンド染色の低減のため吸収処理済み。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A2429-VAR:
A2429-.5ML:
A2429-.25ML:
A2429-1ML:
A2429-BULK:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Xiaogang Wang et al.
Nature communications, 9(1), 1379-1379 (2018-04-13)
Secretion of extracellular vesicles (EVs), a process common to eukaryotes, archae, and bacteria, represents a secretory pathway that allows cell-free intercellular communication. Microbial EVs package diverse proteins and influence the host-pathogen interaction, but the mechanisms underlying EV production in Gram-positive
Antibody structure, instability, and formulation
Wang W, et al.
Journal of Pharmaceutical Sciences, 91(6), 252-265 (2007)
Marcel Zocher et al.
International immunology, 15(7), 789-796 (2003-06-17)
Antigen-specific B cells are key players in many autoimmune diseases through the production of autoreactive antibodies that can cause severe tissue damage and malfunction. We have designed and expressed a fusion protein, referred to as MOG-Fc, composed of the extracellular
The specificity of targeted vaccines for APC surface molecules influences the immune response phenotype
Grodeland G, et al.
Testing, 8(11), e80008-e80008 (2013)
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