A9316

抗マウスIgG (全分子)−アルカリフォスファターゼ ヤギ宿主抗体

affinity isolated antibody, buffered aqueous glycerol solution

• 別名: Goat Anti-Mouse IgG (whole molecule)−AP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162640
• Conjugate: alkaline phosphatase conjugate
• Clone: polyclonal
• Application: ELISA (d), IHC (p), WB
• Citations: 31

特性

• biological source: goat
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• technique(s): direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

詳細

General description

Goat polyclonal anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody is reactive with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM. Cross reactivity of the antibody conjugate is determined by ELISA. The conjugate shows no reactivity with rat IgG or rat serum proteins.

Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant immunoglobulin present in human serum. The four classes of IgG are IgG1, IgG2, IgG3, and IgG4.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

Application

アルカリホスファターゼ抱合ヤギ抗-マウスIgGを、最終濃度0.5MのNaClを含むTBS/Tween中の濃度μg/mLで2次的に用いて、サーファクタントプロテインAが気管支肺胞液中に検出されました。

Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in goat has been used as a secondary antibody in:

• histological studies in heart and liver grafts
• immunohistochemical studies of gangliosides and immunostaining of lymphoid organs
• western blot detection in blood samples

The level of antibodies specific to P. aeuginosa present in immunized mouse sera was determined by ELISA using alkaline phosphatase-conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:1000.

Biochem/physiol Actions

IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.

Physical form

0.05 M Tris溶液(pH 8.0, 10 mMグリシン, 1% BSA, 1 mM MgCl₂, 50%グリセロ-ル, 15 mMアジ化ナトリウム含有)。

Preparation Note

バックグラウンド低減のため、ラットサンプルで吸収済みです。

Other Notes

ラット血清タンパクで吸収済みの抗体です。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable