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Merck

51280

Sigma-Aldrich

ヘミン

BioXtra, from Porcine, ≥96.0% (HPLC)

別名:

クロロ(プロトポルフィリナト)鉄(III), クロロプロトポルフィリンIX鉄(III), クロロヘミン, ヘミン(クロリド), 塩化フェリプロトポルフィリンIX

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1 G
¥12,300
5 G
¥62,400
25 G
¥230,000

¥12,300


在庫あり詳細


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サイズを選択してください

表示を変更する
1 G
¥12,300
5 G
¥62,400
25 G
¥230,000

About This Item

実験式(ヒル表記法):
C34H32ClFeN4O4
CAS番号:
分子量:
651.94
Beilstein:
5229914
EC Number:
MDL番号:
UNSPSCコード:
12352202
PubChem Substance ID:
NACRES:
NA.79

¥12,300


在庫あり詳細


バルクの問い合わせ

由来生物

Porcine

品質水準

製品種目

BioXtra

アッセイ

≥96.0% (HPLC)

フォーム

powder or crystals

テクニック

tissue culture: suitable
transfection: suitable

保管温度

2-8°C

SMILES記法

Cc1c(CCC(O)=O)c2cc3c(CCC(O)=O)c(C)c4cc5nc(cc6c(C)c(C=C)c(cc1n2)n6[Fe](Cl)n34)c(C=C)c5C

InChI

1S/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;

InChI Key

BTIJJDXEELBZFS-HXFTUNQESA-K

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アプリケーション

ヘミンは、
  • 細菌株と増殖条件の研究のため[1]
  • 短期コロニー・アッセイに使用するため(培地は他の成分とともにヘミンで構成)[2]
  • 細胞トランスフェクションの用途に使用されます。[3]
β-ヘマチンの形成に対するMMV (Medicines for Malaria Venture)マラリア・ボックス化合物の阻害活性を分析するためのβ-ヘマチン形成アッセイに使用できます。[4]

生物化学的/生理学的作用

ヘムの酸化形であるヘミンは、さまざまなタンパク質群の鉄含有補欠分子族です。溶血後にヘモグロビンから放出されることがある遊離ヘムは炎症誘発性を有し、鉄由来の活性酸素種に寄与します。遊離ヘミン・レベルは、さまざまな病理学的状態で上方制御されることがあり、血管障害、腎不全、免疫媒介性障害などのさまざまな炎症状態を引き起こします。

ヘミンは、ヘム・オキシゲナーゼ1 (HO-1)の発現と抑制およびHO-1活性の研究に使用できます。 ヘマチンは、インフルエンザ菌の増殖に必要な「X因子」です。

関連製品

製品番号
詳細
価格

保管分類コード

11 - Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, type N95 (US)


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

51280-1G:
51280-25G:
51280-VAR:
51280-BULK:
51280-5G:


最新バージョンのいずれかを選択してください:

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Lot/Batch Number

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Christine C Chiu et al.
PloS one, 14(5), e0216753-e0216753 (2019-05-10)
The mountain pine beetle (MPB; Dendroctonus ponderosae) is a forest insect pest that attacks several different pine (Pinus) species in its native range of distribution in western North America. MPB are exposed for most of their life cycle to the
A Curti et al.
Journal of immunology (Baltimore, Md. : 1950), 166(2), 848-854 (2001-01-06)
We studied cytokine-driven differentiation of primitive human CD34(+)HLA-DR(-) cells to myeloid dendritic cells (DC). Hemopoietic cells were grown in long-term cultures in the presence of various combinations of early acting cytokines such as FLT3-ligand (FLT3-L) and stem cell factor (SCF)
Kim Y Fong et al.
International journal for parasitology. Drugs and drug resistance, 5(3), 84-91 (2015-07-08)
The Malaria Box, assembled by the Medicines for Malaria Venture, is a set of 400 structurally diverse, commercially available compounds with demonstrated activity against blood-stage Plasmodium falciparum. The compounds are a representative subset of the 20,000 in vitro antimalarials identified from
Sophie Joly et al.
Journal of clinical microbiology, 42(3), 1024-1029 (2004-03-09)
Human beta-defensins 2 and 3 (HBD-2 and HBD-3) are inducible peptides present at sites of infection in the oral cavity. A few studies have reported broad-spectrum antimicrobial activity for both peptides. However, no comprehensive study has thoroughly investigated their potential
Eyal Golub et al.
The Analyst, 136(21), 4397-4401 (2011-09-02)
Hemin/G-quadruplex catalyzes the H(2)O(2)-mediated oxidation of Amplex Red to the fluorescent product resorufin. This process is implemented to develop hairpin nucleic acid structures for the detection of DNA, to probe the catalytic activity of glucose oxidase, to use the thrombin-aptamer

質問

1–7/7 質問  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      役に立ちましたか?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 回答
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      役に立ちましたか?

  3. What is the solubility of Hemin, product catalog # 51280, CAS 16009-13-5?

    1 回答
    1. The product with code 51280 can be dissolved in DMSO at a concentration of 1 mM. It is also soluble in 1.4 M ammonium hydroxide at a concentration of 50 mg/ml, and this can be achieved by heating the solution at 95°C for 5 minutes, resulting in a dark brown solution. Additionally, a stock solution of 0.25% hemin in 50% triethanolamine can be prepared. CAS number 16009-13-5, which corresponds to product 51280, is soluble in 1.4 M NaOH at a concentration of 25 mg/mL, and it is practically insoluble in water.

      役に立ちましたか?

  4. How can hemin be dissolved for cell culture experiments in macrophages? Is it necessary to couple it to serum/BSA?

    1 回答
    1. This product has not been tested for use in cell culture. However, a previously deleted product, H1652, has been tested. The H1652 was dissolved at 10 mg/mL in 1 M NaOH and added then added to the culture medium at 7 mg/L. This information would need to be validated for product 51280.

      役に立ちましたか?

  5. How stable are stock solutions of hemin (dissolved in 1N NaOH and diluted to 1 mM with dH2O)? Can they be stored at 4C or -20C?

    1 回答
    1. The solution stability has not been determined.

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  6. What is the Department of Transportation shipping information for this product?

    1 回答
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      役に立ちましたか?

  7. What is the solubility of Product 51280, Hemin?

    1 回答
    1. Hemin can be dissolved in DMSO at 1 mM. This product should be soluble in 1.4 M ammonium hydroxide (50 mg/ml) with 5 minutes of heating at 95 °C, yielding a dark brown solution.  A stock solution of 0.25% hemin in 50% triethanolamine can be made.

      役に立ちましたか?

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