30154

アルキンシアニン色素718

for copper catalyzed click labeling

• 別名: 2-{2-[3-{2-[1,3-ジヒドロ-3,3-ジメチル-1-(4-スルホブチル)-2H-インドール-2-イリジン]エチリジン]}-2-(2-プロピン-1-イルアミノ)-1-シクロペンテン-1-イル]エテニル}-3,3-ジメチル-1-(4-スルホブチル)-3H-インドリウム 分子内塩
• 実験式（ヒル表記法）: C₄₀H₄₉N₃O₆S₂
• CAS番号: 1188292-54-7
• 分子量: 731.96
• MDL番号: MFCD18452828
• UNSPSCコード: 12352200
• PubChem Substance ID: 329753444
• NACRES: NA.32

特性

• 蛍光検出: λ_ex 672 nm; λ_em 718 nm in ethanol
• 保管温度: −20°C
• SMILES記法: CC1(C)\C(=C/C=C2\CCC(\C=C\C3=[N+](CCCCS([O-])(=O)=O)c4ccccc4C3(C)C)=C2NCC#C)N(CCCCS(O)(=O)=O)c5ccccc15
• InChI: 1S/C40H49N3O6S2/c1-6-25-41-38-30(21-23-36-39(2,3)32-15-7-9-17-34(32)42(36)26-11-13-28-50(44,45)46)19-20-31(38)22-24-37-40(4,5)33-16-8-10-18-35(33)43(37)27-12-14-29-51(47,48)49/h1,7-10,15-18,21-24H,11-14,19-20,25-29H2,2-5H3,(H2,44,45,46,47,48,49)/b30-21+,36-23+
• InChI Key: YNKZQYLIDKIPBB-GBYGALLISA-N

詳細

詳細

The MegaStokes dyes for „click" biological labeling, licensed by LuminoChem are virtually covering the whole visible spectrum reaching the infra-red regime. Besides dyes that are being capable of participating in classical copper catalyzed 1,3-dipolar cycloaddition reaction with the counterparting function, we also provide dyes containing cyclooctyne moiety an alkyne derivative that enables copper free clicking to azides.
These dyes are noteworthy for their large Stokes-shift (120 or 125 nm). Dyes for this kind are exceptionally useful in fluorescence resonance electron transfer (FRET) applications as they do not interfere with the spectral bands of the second fluorophore a common problem in FRET technology. Azido sugars have been used for click-labeling of surface glycoproteins, recently. We have adapted this system to demonstrate the ability of our dyes to undergo bioorthogonal labeling reaction, efficiently. Prior to labeling, Chinese hamster ovary (CHO) cells were incubated with these MegaStockesdyes to test the possible cytostatic effects of these dyes. Experimental results have shown that neither dye is toxic up to 50 μM concentration.

アプリケーション

Alkyne cyanine dye 718 is a reagent for copper catalyzed click labeling.

包装

底の開いたガラス瓶内容物は内部に挿入され接着された円錐部に入っています。

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable