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安全性情報

00062107

MDCK-II細胞株 イヌ

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

別名:

MDCK Type II Cells, MDCK-2 Cells, MDCKII-WT Cells

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About This Item

UNSPSCコード:
41106514
NACRES:
NA.81
現在、価格および在庫状況を閲覧できません。

由来生物

canine kidney

品質水準

100

詳細

Canine Cocker Spaniel Kidney

フォーム

liquid

包装

tube of 5 μg 00062107-DNA-5UG
pkg of vial of cells 00062107-1VL

成長モード

Adherent

核型

Dog

形態

Polarised epithelial

製品

Not specified

受容体

Not specified

テクニック

cell culture | mammalian: suitable

細胞株の由来

Canine Cocker Spaniel Kidney

細胞株の説明

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (Sigma catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. Mycoplasma eradicated using Boehringer method prior to deposit at ECACC.

包装

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

培地

EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 5% FBS (F2442)

継代と培養方法

Split sub-confluent cultures (70-80%) 1:5 twice a week i.e. seeding at 5 x 10,000 cells/cm2 using 0.25% tryspin or trypsin/EDTA; 5% CO2 37°C,

その他情報

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

保管分類コード

10 - Combustible liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

労働安全衛生法名称等を表示すべき危険物及び有害物

名称等を表示すべき危険物及び有害物

労働安全衛生法名称等を通知すべき危険物及び有害物

名称等を通知すべき危険物及び有害物

Jan Code

00062107-1VL:

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試験成績書（COA）

Lot/Batch Number

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Manuel S Brugger et al.
Biosensors & bioelectronics, 173, 112807-112807 (2020-11-23)
A Love-wave based biosensor is introduced for analyzing a standardized wound healing assay by observing cell growth and quantifying cell detachment processes. Utilizing the piezoelectric material LiTaO3 36° XY-cut with a thin SiO2-cover layer, shear horizontal surface acoustic waves (SAW)
Olga Danov et al.
Frontiers in medicine, 7, 571003-571003 (2020-11-27)
Smokers with apparently "healthy" lungs suffer from more severe and frequent viral respiratory infections, but the mechanisms underlying this observation are still unclear. Epithelial cells and dendritic cells (DC) form the first line of defense against inhaled noxes such as
D Louvard
Proceedings of the National Academy of Sciences of the United States of America, 77(7), 4132-4136 (1980-07-01)
A dog kidney epithelial cell line (MDCK), grown in monolayer, displayed in vitro an asymmetric localization of surface proteins. Aminopeptidase [alpha-aminoacylpeptide hydrolase (microsomal), EC 3.4.11.2] was found only in the apical face whereas Na+, K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) was

資料

Cellular Fluorescence Imaging with Millicell® Cell Culture Inserts

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

質問

  1. Dear ECACC, We are currently developing critical assays using this MDCK-II cell line (ECACC 00062107) at Genentech. I hope to ask your help to get any gene or protein expression database of this cell line. Any published data available? Thank you!

    1 回答

    1. This cell line is a product of ECACC. Please see the information below to review the ECACC product and contact information, original citation, and depositor.

      ECACC MDCK II product page:
      https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=00062107&collection=ecacc_gc

      ECACC contact form:
      https://www.culturecollections.org.uk/contactus/index.aspx

      Link to original cited reference:
      https://pubmed.ncbi.nlm.nih.gov/3519211/

      Cell Line Depositor:
      Professor Gerrit van Meer, Dept. of Membrane Enzymology, Centre for Biomembranes and Lipid Enzymology, H.R Kruytgebouw N607, Padualaan 8, 3584 CH Utrecht, The Netherlands.

      役に立ちましたか？

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