PPB023

Tris buffer

Pre-set, pH 8.1, pHast Pack^™, powder

• 別名: 0.1M Tris
• UNSPSCコード: 12161700
• NACRES: NA.25

特性

• 品質水準: 100
• 製品種目: pHast Pack^™
• 形状: powder
• 保管条件: (Tightly closed. Dry)
• 不純物: ≤5 ppm Heavy Metals (lead)
• 色: white
• pH: 8.1
• 微量陽イオン: Fe: ≤10 ppm
• その他の活性: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• 保管温度: room temp

詳細

詳細

Tris buffer, pH 8.1 is a general biological buffer commonly used in biochemistry and molecular biology. It is isotonic and non-toxic in nature due to the presence of tris base. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

アプリケーション

Intended for use in:

• Cell culture (sterile filter recommended)
• Western blot
• Protein electrophoresis
• DNA agarose electrophoresis as a component to make Tris acetate-EDTA (TAE) and Tris borate-EDTA (TBE) buffers

特徴および利点

• Ready-to-use for quick buffer prep
• No measuring and adjusting pH
• Saves time and minimizes effort in the lab
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

包装

Foil pouches

再構成

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 8.1 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

保管および安定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

その他情報

For additional information on our range of Biochemicals, please complete this form.

法的情報

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1