It is known that ionic contrast media (CM) bind magnesium and that patients with asthma often benefit from magnesium injections. Similarly, asthmatics have a higher incidence of CM reactions. This work tests the hypothesis that ionic CM alter serum magnesium
Intracellular free Mg2+ (Mgf) in darkness and during illumination was measured in the Balanus photoreceptor using the Mg2+ sensitive dye Eriochrome Blue SE (EB). The EB was pressure injected in the cell and Mgf determined from measurements of absorption changes
Cytoplasmic, free Mg2+ was measured spectrophotometrically using an intrasynaptosomally entrapped Mg2+-indicator, Eriochrome blue (EB). Addition of the ionophore A23187 or disruption of the synaptosomal plasma membrane with digitonin caused an increase in absorbance of entrapped EB with a maximum at
Measurement of ionized magnesium in biological fluids.
A nondestructive technique is described for measuring intracellular free Mg2+ concentrations. It is based on the microinjection of a Mg2+ metallochromic indicator in the cytosol of a large single cell and measuring its differential absorbance changes with sensitive multiple wavelength