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形状
solution
品質水準
包装
pkg of 500 μL (10 μg/ml)
メーカー／製品名
Roche
環境により配慮した代替製品の特徴
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
環境により配慮した代替製品カテゴリ
保管温度
−20°C
詳細
DNA Molecular Weight Marker VI, DIG-labeled is a fragment mixture prepared by cleavage of pBR328 DNA and pBR328 DNA with restriction endonucleases Bgl I and Hinf I respectively. The size of the fragment ranges from 0.15 to 2.1 kbp.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
アプリケーション
DNA Molecular Weight Marker VI, DIG-labeled, has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection. It has also been used as a size standard for amplified DNA.
構成
Ready-to-use solutions in 10mM Tris-HCl, 1mM EDTA, pH 8.0.
シーケンス
The mixture contains 12 fragments wit the following base pair lengths: 154/154, 220, 234/234, 298/298, 394, 453, 517, 653, 1033, 1230, 1766, and 2176 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
その他情報
12 fragments: 154/154; 220; 234/234; 298/298; 394; 453; 517; 653; 1,033; 1,230; 1,766; 2,176 bp
Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
For life science research only. Not for use in diagnostic procedures.
保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
No data available
引火点(℃)
No data available
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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M Agüero et al.
Journal of clinical microbiology, 41(9), 4431-4434 (2003-09-06)
This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A
R Ferretti et al.
Applied and environmental microbiology, 67(2), 977-978 (2001-02-07)
A PCR-based method for the detection of Salmonella spp. in food was developed. The method, set up on typical salami from the Italian region of Marche, is sensitive and specific and shows excellent correlation with the conventional method of reference
Stéphanie Tomé et al.
PLoS currents, 6, doi:10-doi:10 (2014-03-13)
Many human diseases are associated with the abnormal expansion of unstable trinucleotide repeat sequences. The mechanisms of trinucleotide repeat size mutation have not been fully dissected, and their understanding must be grounded on the detailed analysis of repeat size distributions
資料
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
DNAおよびRNA DIGプローブ用のジゴキシゲニン（DIG）による標識方法とキット、ランダムプライミングによるDNA標識、ニックトランスレーションによる標識、5'および3'オリゴヌクレオチド末端標識。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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